Optical Analysis of Synaptic Integration in Cerebellar Purkinje Cells

为了揭示学习记忆的机制， 了解突触输入的方式至关重要， 在神经元中整合和加工，特别是在树突状细胞中 水平 最近，已经开发了光学技术， 可以测量膜电位和细胞内 钙同时从许多地方，包括罚款 树突状突起 这个项目将使用这些新技术 为了检查浦肯野细胞的电活动， 豚鼠小脑 特别是，实验将 进行观察a）空间和时间特征 爬行纤维和颗粒细胞神经元的接触 浦肯野细胞神经元，和B）不同的相互作用， 神经元之间的联系 的计算机模型 浦肯野细胞也将被开发，可用于帮助 解释光学数据。

In order to reveal the mechanisms of learning and memory it is essential to have information about the way synaptic inputs are integrated and processed in a neuron, especially at the dendritic level. Recently, optical techniques have been developed which can measure changes in membrane potential and intracellular calcium simultaneously from many locations, including fine dendritic processes. This project will use these new techniques to examine electrical events in Purkinje cells in slices from the guinea pig cerebellum. In particular, experiments will be performed to look at a) the spatial and temporal characteristics of climbing fiber and granule cell neuronal contacts with Purkinje cell neurons, and b) the interaction of the different interneuronal contacts with each other. A computer model of the Purkinje cell will also be developed which can be used to help interpret the optical data.