Optical Analysis of Synaptic Integration in Cerebellar Purkinje Cells

小脑浦肯野细胞突触整合的光学分析

• 批准号: 9209784
• 负责人: William Ross
• 金额: $ 20.69万
• 依托单位: New York Medical College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9209784&HistoricalAwards=false
• 关键词: Optical; Analysis; Synaptic; Integration; Cerebellar

This application makes use of state-of-the-art imaging techniques to spatially and temporally resolve the propagation of specific ionic transients. Dr. Ross will investigate this problem by using a relatively noninvasive high speed imaging system which can examine all parts of the cell simultaneously, This optical technique can give information about specific ion concentration changes and voltage changes. The signals can be interpreted in terms of the spatial distribution and propagation of dendritic events and the distribution of specific membrane channels. Dr. Ross will inject individual Purkinje cells in cerebellar slices with fluorescent ion indicators and high speed images, correlated with simultaneous electrical measurements made under various conditions. The special imaging technology will be specifically used for measuring intracellular calcium and sodium concentration transients in fine dendritic processes. These experiments will attempt to define those factors (intrinsic membrane conductances, synaptic interactions, and neuromodulators) which influence the generation of local intracellular calcium changes and the propagation of regenerative potentials. Because it is essential to have information about the way synaptic inputs are integrated and processed in a neuron results from this study will ultimately help to better understand the cellular mechanisms of learning and memory.***

该应用程序使用了最先进的成像技术 以在空间和时间上解决特定病毒的传播 离子瞬变 罗斯博士将通过使用 一种相对无创的高速成像系统， 同时检查细胞的所有部分，这种光学 技术可以给出关于特定离子浓度信息 变化和电压变化。 这些信号可以解释为 枝晶的空间分布和传播 事件和特定膜通道的分布。 博士 罗斯会将单个浦肯野细胞注入小脑切片 用荧光离子指示剂和高速图像， 同时在各种条件下进行电测量 条件 这种特殊的成像技术将专门 用于测量细胞内钙和钠浓度 精细树枝状过程中的瞬态。 这些实验将 试图定义这些因素（固有膜电导， 突触相互作用和神经调质），这些因素影响 产生局部细胞内钙变化， 再生潜能的传播。 因为这是必不可少的 了解突触输入的整合方式 并在神经元中进行处理， 有助于更好地理解学习的细胞机制， 记忆。*