Reversed Phase Chromatography in a Magnetically Stabilized Fluidized Bed

磁稳定流化床反相色谱

• 批准号: 8820150
• 负责人: David Graves
• 金额: $ 9.5万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-03-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8820150&HistoricalAwards=false
• 关键词: Reversed; Phase; Chromatography; Magnetically; Stabilized

Fixed-bed operations are central to many separation and purification processes; a critical factor limiting efficiency in many cases is the excess pressure drop which develops as particle size decreases and bed depth increases. One way of preventing this is to fluidize the bed; fluidization has the drawback, however, of increasing the mixing between volume elements in the bed, which reduces chromatographic separation of mixture components. Stabilization of the bed with a magnetic field can improve the separation. A critical area for investigation of the potential advantages of using a magnetically stabilized fluidized bed is that of large-scale reverse phase chromatography. Reverse phase chromatography is an important purification process in many schemes for producing commercial quantities of biological molecules produced from genetically altered microorganisms or cell-cultures. This project will examine potential improvements to the efficiency of reverse phase chromatography for bioseparations from its implementation in a magnetically stabilized fluidized bed. Lower pressure drops, better ability to handle entrained particulates, and possible improvements in packing handling ability in an MSFB configuration make this a potentially important process for downstream processing in the biotechnology industry.

固定床操作是许多分离和提纯过程的核心；在许多情况下，限制效率的关键因素是随着颗粒尺寸减小和床层深度增加而发展的过剩压降。防止这种情况的一种方法是使床层流态化；然而，流态化的缺点是增加了床层中体积元素之间的混合，从而减少了混合物组分的色谱分离。用磁场稳定床层可以改善分离效果。研究使用磁稳定流态化床的潜在优势的一个关键领域是大规模反相色谱。反相层析是从转基因微生物或细胞培养中生产商业批量生物分子的许多方案中的重要纯化过程。该项目将考察反相色谱分离生物的效率与其在磁稳定的流态化床中的实施相比的潜在改进。更低的压降、更好的处理夹带颗粒的能力，以及MSFB配置中填料处理能力的可能改进，使其成为生物技术行业下游加工的潜在重要工艺。