Reversed Phase Chromatography in a Magnetically Stabilized Fluidized Bed

固定床操作是许多分离和净化过程的核心；在许多情况下，限制效率的一个关键因素是随着粒径的减小和床层深度的增加而产生的超压降。防止这种情况的一种方法是使床流化；然而，流化的缺点是增加了床中体积元素之间的混合，从而减少了混合物组分的色谱分离。利用磁场稳定床层可以提高分离效果。研究磁稳定流化床潜在优势的一个关键领域是大规模反相色谱。反相色谱法是许多从转基因微生物或细胞培养物中生产商业数量生物分子的方案中重要的纯化过程。本项目将研究在磁稳定流化床中实施反相色谱法对生物分离效率的潜在改进。更低的压降，更好的处理夹带颗粒的能力，以及在MSFB配置中包装处理能力的可能改进，使其成为生物技术行业下游处理的潜在重要工艺。

Fixed-bed operations are central to many separation and purification processes; a critical factor limiting efficiency in many cases is the excess pressure drop which develops as particle size decreases and bed depth increases. One way of preventing this is to fluidize the bed; fluidization has the drawback, however, of increasing the mixing between volume elements in the bed, which reduces chromatographic separation of mixture components. Stabilization of the bed with a magnetic field can improve the separation. A critical area for investigation of the potential advantages of using a magnetically stabilized fluidized bed is that of large-scale reverse phase chromatography. Reverse phase chromatography is an important purification process in many schemes for producing commercial quantities of biological molecules produced from genetically altered microorganisms or cell-cultures. This project will examine potential improvements to the efficiency of reverse phase chromatography for bioseparations from its implementation in a magnetically stabilized fluidized bed. Lower pressure drops, better ability to handle entrained particulates, and possible improvements in packing handling ability in an MSFB configuration make this a potentially important process for downstream processing in the biotechnology industry.