Reversed Phase Chromatography in a Magnetically Stabilized Fluidized Bed

固定床操作是许多分离和纯化的核心 在许多情况下，限制效率的关键因素是 随着颗粒尺寸减小和床层厚度的增加， 深度增加。 防止这种情况的一种方法是把床铺好; 然而，流化具有增加混合的缺点， 在床中的体积元素之间，这减少了色谱 混合物组分的分离。 床的稳定性， 磁场可以改善分离效果。 一个关键领域的调查的潜在优势， 磁稳定流化床是一种大型的逆向流化床， 相色谱法 反相色谱法是一种重要的 在许多用于生产商业产品的方案中， 大量的生物分子产生于基因改变 微生物或细胞培养物。 该项目将研究潜在的 改进反相色谱法的效率， 生物分离从它的实施在磁稳定 流化床 更低的压降，更好的处理能力 夹带的颗粒，以及可能改进的包装处理 MSFB配置中的功能使其成为一个潜在的重要 在生物技术工业中的下游加工工艺。

Fixed-bed operations are central to many separation and purification processes; a critical factor limiting efficiency in many cases is the excess pressure drop which develops as particle size decreases and bed depth increases. One way of preventing this is to fluidize the bed; fluidization has the drawback, however, of increasing the mixing between volume elements in the bed, which reduces chromatographic separation of mixture components. Stabilization of the bed with a magnetic field can improve the separation. A critical area for investigation of the potential advantages of using a magnetically stabilized fluidized bed is that of large-scale reverse phase chromatography. Reverse phase chromatography is an important purification process in many schemes for producing commercial quantities of biological molecules produced from genetically altered microorganisms or cell-cultures. This project will examine potential improvements to the efficiency of reverse phase chromatography for bioseparations from its implementation in a magnetically stabilized fluidized bed. Lower pressure drops, better ability to handle entrained particulates, and possible improvements in packing handling ability in an MSFB configuration make this a potentially important process for downstream processing in the biotechnology industry.