Evaluation of Gene Probe Technology for the Detection of Human Immunodeficiency Virus in Hospital Wastewater Concen- trates

医院废水浓缩物中人类免疫缺陷病毒检测基因探针技术的评价

• 批准号: 8905210
• 负责人: Joan Rose
• 金额: $ 3万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-01 至 1990-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8905210&HistoricalAwards=false
• 关键词: Evaluation; Gene; Probe; Technology; Detection

The objective of this project is to conduct exploratory research on filtration techniques for detection and recovery of the human immunodeficiency virus (HIV) and on the use of gene probe techniques for detection of the presence of this virus in water that has been in contact with solid and liquid wastes that are contaminated with this virus. In this preliminary research, recovery of the virus will be investigated by use of positively charged depth filters, a technique that has been shown to absorb virus particles at or nearly a neutral pH. Positive results of detection procedures will be confirmed by use of tissue culture when applicable or by restriction analysis and subsequent gel electrophoresis to visualize the viral constituents or metabolic products. This project is being supported under the NSF Program of Expedited Awards for Novel Research. Results of this project are expected to assist in determination of the impact that potential contamination of water by the HIV-virus can have on the engineering design of processes and systems for disinfection and decontamination of environmental resources.

该项目的目的是进行探索性的 过滤技术的研究， 人体免疫机能丧失病毒（艾滋病毒）的康复， 使用基因探针技术检测 这种病毒在水中的存在， 接触固体和液体废物， 感染了这种病毒 在这个初步的 研究，病毒的恢复将由 使用带正电荷的深度过滤器， 已经被证明可以吸收病毒颗粒 pH值接近中性，检测结果呈阳性 将通过使用组织培养来确认程序 适用时或通过限制性分析， 随后进行凝胶电泳以观察病毒 成分或代谢产物。 该项目得到了NSF计划的支持 小说研究加速奖 结果 该项目预计将有助于确定 水的潜在污染的影响， 艾滋病毒可以对工程设计 用于消毒的方法和系统， 净化环境资源。