Evaluation of Gene Probe Technology for the Detection of Human Immunodeficiency Virus in Hospital Wastewater Concen- trates

该项目的目标是对检测和恢复人类免疫缺陷病毒(HIV)的过滤技术进行探索性研究，并使用基因探针技术检测与下列固体和液体废物接触的水中是否存在这种病毒感染了这种病毒。在这项初步研究中，将使用带正电的深度过滤器来研究病毒的恢复，这是一种已被证明在pH值或接近中性的条件下吸收病毒颗粒的技术。检测程序的阳性结果将通过适当的组织培养或通过限制性内切酶分析和随后的凝胶电泳法来确认，以显示病毒成分或代谢产物。该项目得到了美国国家科学基金会新奇研究快速奖项目的支持。预计该项目的结果将有助于确定艾滋病毒病毒对水的潜在污染可能对环境资源消毒和净化程序和系统的工程设计产生的影响。

The objective of this project is to conduct exploratory research on filtration techniques for detection and recovery of the human immunodeficiency virus (HIV) and on the use of gene probe techniques for detection of the presence of this virus in water that has been in contact with solid and liquid wastes that are contaminated with this virus. In this preliminary research, recovery of the virus will be investigated by use of positively charged depth filters, a technique that has been shown to absorb virus particles at or nearly a neutral pH. Positive results of detection procedures will be confirmed by use of tissue culture when applicable or by restriction analysis and subsequent gel electrophoresis to visualize the viral constituents or metabolic products. This project is being supported under the NSF Program of Expedited Awards for Novel Research. Results of this project are expected to assist in determination of the impact that potential contamination of water by the HIV-virus can have on the engineering design of processes and systems for disinfection and decontamination of environmental resources.