Design of Environmentally Sensitive Fluorescent Nucleosides and Their Application to Innovative Fluorescence Probe for Gene Analysis

环境敏感荧光核苷的设计及其在基因分析创新荧光探针中的应用

• 批准号: 23350082
• 负责人: SAITO Isao
• 金额: $ 12.48万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23350082/
• 关键词: DNA プローブ; DNAデバイス; 蛍光分子; ナノバイオ; 環境応答分子; 遺伝子診断; DNAチップ

We have designed a novel type of base-discriminating fluorescent (BDF) nucleosides which are used for SNP genotyping and gene detection. We also succeeded to design environmentally sensitive fluorescent (ESF) nucleosides that emit strong fluorescence at different wavelengths depending upon a difference of local environment near the ESF base. The newly designed ESF nucleosides may discriminate structural changes such as single strands, matches, mismatches and deletions when incorporated in DNA by a significant change of emission wavelengths as well as their intensities. Quencher and end free molecular beacons (MB) were discovered for the first time by using newly designed ESF nucleosides. These low cost MB were used for the gene detection in a cell. ESF nucleosides that are sensitive to pH were also designed. Such pH sensor ESF nucleoside that emits strong fluorescence only at bellow pH 5.5 was used for the detection of cancer cell.

我们设计了一种新型的碱基识别荧光（BDF）核苷，用于SNP基因分型和基因检测。我们还成功地设计了环境敏感的荧光（ESF）核苷，根据ESF碱基附近的局部环境的差异，在不同的波长下发射强荧光。新设计的ESF核苷可以区分结构变化，如单链，匹配，错配和缺失，当掺入DNA时，通过发射波长及其强度的显着变化。利用新设计的ESF核苷首次发现了猝灭剂和末端自由分子信标（MB）。 这些低成本的MB用于细胞中的基因检测。 还设计了对pH敏感的ESF核苷。 这种pH传感器ESF核苷仅在pH 5.5以下发射强荧光，用于检测癌细胞。

项目成果

Design and Synthesis of Highly Solvatochromic Fluorescent 2 `-Deoxyguanosine and Adenosine Analogs

高溶剂化显色荧光2`-脱氧鸟苷和腺苷类似物的设计与合成

• 发表时间: 2011
• 期刊: Bioorg.Med.Chem Lett
• 作者: K. Matsumoro;N. Takahashi;A. Suzuki;T. Morii;Y. Saito and I. Saito
• 通讯作者: Y. Saito and I. Saito

pH変化に敏感なアントラセン骨格を有する蛍光性ヌクレオシドの開発と応用

pH变化敏感的蒽骨架荧光核苷的开发及应用

• 发表时间: 2012
• 作者: Ryo Tsunashima;Takumi Matsumoto;Kei Kondo;Yoshihiko Suyama;Yukihiro Nishioka;Jun Kawamata;Shin-ichiro Noro;Takayoshi Nakamura;Tomoyuki Akutagawa ;Katsuya Ishiguro;鈴木梓・宮本茂憲・横山祥太・石原務・ 齋藤烈・根本修克・ 齋藤義雄
• 通讯作者: 鈴木梓・宮本茂憲・横山祥太・石原務・ 齋藤烈・根本修克・ 齋藤義雄

Fluorescent nucleosides with 'on-off' switching function, pH-responsive fluorescent uridine derivatives.

• DOI: 10.1016/j.bmcl.2012.02.092
• 发表时间: 2012-04
• 期刊: Bioorganic & medicinal chemistry letters
• 影响因子: 2.7
• 作者: Yoshio Saito;S. Miyamoto;Azusa Suzuki;Katsuhiko Matsumoto;T. Ishihara;I. Saito

Fluorometric Detection of Adenine in Target DNA by Exciplex Formation with Fluorescent 8-Arylethynylated Deoxyguaanosine .

通过与荧光 8-芳基乙酰化脱氧鸟苷形成激基复合物来荧光检测目标 DNA 中的腺嘌呤。

• 发表时间: 2012
• 期刊: Tetrahedron Lett.
• 作者: Y. Saito;K. Kugenuma;M. Tanaka;A. Suzuki;and I. Saito,
• 通讯作者: and I. Saito,

Design and Synthesis of Highly Solvatochromic Fluorescent 2'-Deoxyguanosine and Adenosine Analogs

高度溶剂化显色荧光 2-脱氧鸟苷和腺苷类似物的设计与合成

• 发表时间: 2011
• 期刊: Bioorg.Med.Chem.Lett.
• 作者: K.Matsumoro;N.Takahashi;A.Suzuki;T.Morii;Y.Saito;I.Saito
• 通讯作者: I.Saito