Specific Antibody Production in Plant Cell Cultures

这项拟议的研究的目标是开发一种大规模的工艺，通过使用改良的植物细胞来生产功能性哺乳动物抗体蛋白。建立能够合成功能性抗体蛋白的转化植物组织培养细胞，对于廉价高效地生产大量特异性抗体具有巨大的潜力。将单域抗溶菌酶抗体cDNA亚克隆到植物表达载体中，并导入培养的植物细胞中，用于生产抗体蛋白。将测试不同的信号肽序列，以有效地将抗体蛋白从培养细胞分泌到培养液中。将进行细胞培养的放大和优化。抗体产生细胞的遗传稳定性将受到监测。

The goal of this proposed research is to develop a large-scale process to produce functional mammalian antibody proteins by employing modified plant cells. The creation of transformed plant tissue culture cells capable of synthesizing functional antibody proteins has great potential for producing large quantities of specific antibodies cheaply and efficiently. Single domain anti-lysozyme antibody cDNAs have been subcloned into a plant expression vector and introduced into cultured plant cells which will be employed for the production of antibody proteins. Various signal peptide sequences will be tested for the efficient secretion of antibody proteins into the medium from cultured cells. The scale-up and optimization of the cell cultivation will be carried out. The genetic stability of the antibody-producing cells will be monitored.