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Parameters Influencing Efficient Expression of Mammalian Proteins in Plant Cell Cultures

Parameters Influencing Efficient Expression of Mammalian Proteins in Plant Cell Cultures
影响植物细胞培养物中哺乳动物蛋白高效表达的参数
批准号：
9308407
负责人：
James Lee
金额：
$0.0万
依托单位：
Washington State University
依托单位国家：
美国
项目类别：
Continuing grant
财政年份：
1993
资助国家：
美国
项目状态：
已结题
起止时间：
1993-09-01 至 1996-02-29
来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9308407&HistoricalAwards=false
关键词：
ParametersInfluencingEfficientExpressionMammalian

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英文摘要
9308407 Lee This project is on research to increase the foreign gene expression level in plant cell cultures. This involves studying the cellular and environmental parameters influencing the efficient production of mammalian proteins in suspension plant cell cultures and obtaining stronger promoter systems than those currently available. Also, an understanding of how different properties of mammalian proteins affect the efficiency of gene expression in plant cell cultures will be pursued. To facilitate this last objective, the proteins in this study include monoclonal antibodies, bovine lysozyme, human interleukin 6 (IL- 6), and the Tac subunit of the interleukin 2 receptor protein (IL - 2R). In addition to having different structural and biochemical properties, these products all have important applications in medicine and biotechnology. ***
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