Acquisition of Light Microscope/Image Analysis System

购买光学显微镜/图像分析系统

• 批准号: 9108059
• 负责人: Curt Peterson
• 金额: $ 1.6万
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-11-15 至 1994-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9108059&HistoricalAwards=false
• 关键词: Acquisition; Light; Microscope; Image; Analysis

This proposal requests funds for the acquisition of a microscope, image analysis system, and computer which will be used by eight scientists working independently or collaboratively on five different projects to expand and technically enhance ongoing basic research in plant molecular, cellular, and developmental biology. This research is experimental in approach and focused on cellular and molecular biological interests, such as, chloroplast dna molecules, ultrastructural responses to heat shock mechanisms controlling abscission, bacterial gene expression, and the formation of pit membranes in woody dicots. An image analysis system capable of performing quantitative morphometric and stereological measurements is essential for the successful completion of the research. Also, proposed research investigating various cellular responses require fluorescence microscopy. For example, immunofluorescence microscopy will be used for the localization of specific cytoskeletal proteins and to visualize and quantify subcellular features associated with target cell differentiation in abscission zones of soybean.

这项提案要求提供资金，用于购置一台显微镜， 图像分析系统和计算机将用于八个 科学家们独立或合作研究五个 不同的项目，以扩大和技术上加强正在进行的 植物分子、细胞和发育的基础研究 生物学 这项研究是实验性的方法和重点 对细胞和分子生物学的兴趣，例如， 叶绿体dna分子，超微结构，热响应 控制解离的休克机制，细菌基因 表达，以及木本双子叶植物中纹孔膜的形成。 一种图像分析系统，能够执行定量分析， 形态学和体视学测量对于 成功完成研究。 此外，拟议的研究 研究各种细胞反应需要荧光 显微镜 例如，免疫荧光显微镜将是 用于定位特定的细胞骨架蛋白， 可视化和量化与以下疾病相关的亚细胞特征 大豆离体区靶细胞分化