Acquisition of an Automated DNA Sequencer

哈佛大学的五位高级教师正在申请应用生物系统公司的373A-01型自动DNA测序仪及其相关的硬件和软件。哈佛大学在分子生物学和进化生物学的新合成方面处于独特的地位，因为它在这两个领域都有悠久的卓越传统。事实上，生命科学学术成长和发展的重点之一是分子生物学和进化生物学之间的重叠。哈佛大学的有机体和进化生物系（OEB）已经在扩大规模，增加了代表这种整合的新教员。在参与的5名OEB教员中，有4名（哈特尔、多诺霍、凯洛格和皮尔斯）是哈佛大学的新教员，3名（哈特尔、多诺霍和皮尔斯）是资深教员。参与小组对自动DNA测序的需求是迫切和迫切的。没有自动DNA测序仪可用于碎片使用。在申请人中，没有一个单独的团体足够大，或者它的需求足够广泛，足以证明购买、维护和单独使用自动测序仪是合理的。然而，总的来说，这些小组基本上可以充分利用自动测序仪（估计测序每年需要大约100万bp）。自动DNA测序仪将安装在Hartl实验室，该实验室位于参与用户的中心，并将由Hartl小组成员Elena Lozovskaya博士监督，她在自动DNA测序方面具有丰富的实践经验。大多数参与小组已经有使用自动DNA测序仪的相关经验，他们的项目得到资助并准备实施。因此，自动DNA测序仪是一种熟悉的设备，将被设置在用户的中心位置，由经验丰富的调查员监督，并立即使用，基本上是满负荷的。OEB将为购买仪器和支持经常性服务合同的主要部分作出贡献。哈佛大学的学生正在申请应用生物系统公司373A-01型自动DNA测序仪​!F $ $ (Times New Roman Symbol & Arial " h E E E + ！沙漠之王；沙漠之王

9317625 Hartl Five senior faculty at Harvard University are applying for an Applied Biosystems Model 373A-01 Automated DNA Sequencer with associated hardware and software. Harvard is in a unique position to lead a new synthesis of molecular biology and evolutionary biology because of its long tradition of excellence in both fields. Indeed, among the priorities for academic growth and development in the life sciences is the overlap between molecular biology and evolutionary biology. The Department of Organismic and evolutionary Biology (OEB) at Harvard is already expanding with new faculty representing this type of integration. Among the five OEB faculty participating, four (Hartl, Donoghue, Kellogg, and Pierce) are new to Harvard, and three (Hartl, Donoghue, and Pierce) are senior appointments. The need for automated DNA sequencing in the participating groups is immediate and acute. There is no automated DNA sequencer available for shard use. Among the applicants, no group alone is large enough or its needs extensive enough to justify purchase, maintenance, and exclusive use of an automated sequencer. However, in the aggregate, the groups can make use of an automated sequencer essentially to full capacity (estimated sequencing needs approximately 1 million bp per year). The automated DNA sequencer will be set up in the Hartl laboratory, which is centrally located among the participating users, and will be supervised by Dr. Elena Lozovskaya, a member of the Hartl group who has extensive practical experience in automated DNA sequencing. Most of the participating groups have already had relevant experience using automated DNA sequencers and their projects are funded and ready to be implement. Therefore, the automated DNA sequencer is a familiar piece of equipment that will be set up in a central location among the users, supervised by an experienced investigator, and used immediately to essentially full capacity. OEB will contribute to the purchase of the instrument and support of the major portion of the recurring service contract. at Harvard University are applying for an Applied Biosystems Model 373A-01 Automated DNA Seq ! F $ $ ( Times New Roman Symbol & Arial " h E E E + ! = abstract Deseree King, BIR Deseree King, BIR