Marine BioTech Post-doc: Coevolution In A Luminous Bacterium-Squid Symbiosis

海洋生物技术博士后：发光细菌-鱿鱼共生的共同进化

• 批准号: 9321645
• 负责人: Edward Ruby
• 金额: $ 9万
• 依托单位: University of Southern California
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-10-01 至 1996-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9321645&HistoricalAwards=false
• 关键词: Marine; BioTech; Post; doc; Coevolution

9321645 Ruby Bioluminescent marine bacteria of the species, Vibrio fischeri, are the light organ symbionts of the sepiolid squid genera Euprymna, and Sepiola. Bacterial colonization of the developing light organ results in the establishment of a successful association by one specific subset (or subspecies) of Vibrio fischeri strains. This project will examine populations of V. Fischeri in several overlapping species of sepiolid squid from two locations, and will determine whether these populations of V. fischeri are host specific. Through the use of PCR amplification, restriction fragment length polymorphism (RFLP), and sequence analysis of both host and symbiont DNA, the phylogenetic relationships both among various strains of V. fischeri and among sepiolid squid species will be inferred. Along with the molecular phylogenetic analysis, cross- colonization experiments will be completed to determine whether the symbiotic bacteria are able to initiate any colonization in other host species of squid. Strains that colonize animals other than their host species, will be tested for the degree, success, and competitive dominance with the natural symbiotic strain. This model is the only known type of animal/bacterial symbioses where both host and symbiont can be cultured successfully and independently from one another, and provides a unique experimental system to address the role of evolution in symbioses. ***

9321645红宝石生物发光海洋细菌的物种，弧菌fischeri，是光器官共生体的乌贼属Euprymna，和乌贼。 发育中的光器官的细菌定殖导致通过费氏弧菌菌株的一个特定子集（或亚种）建立成功的关联。 该项目将检查来自两个地点的几个重叠的乌贼物种中的V. Fischeri种群，并将确定这些V. Fischeri种群是否具有宿主特异性。 通过使用PCR扩增，限制性片段长度多态性（RFLP），和宿主和共生体DNA的序列分析，V. fischeri的各种菌株之间的系统发育关系和乌贼物种之间的推断。 沿着分子系统发育分析，将完成交叉定殖实验，以确定共生细菌是否能够在鱿鱼的其他宿主物种中启动任何定殖。 将测试定殖动物而不是其宿主物种的菌株的程度、成功率和与天然共生菌株的竞争优势。 该模型是唯一已知的动物/细菌共生体类型，其中宿主和共生体可以成功地相互独立地培养，并提供了一个独特的实验系统来解决共生体中进化的作用。 ***