Orientation and Function in Self-Organized Protein Films

自组织蛋白质膜的取向和功能

• 批准号: 9403896
• 负责人: Scott Saavedra
• 金额: $ 31.63万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-15 至 1997-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9403896&HistoricalAwards=false
• 关键词: Orientation; Function; Self; Organized; Protein

This project, jointly supported by the Division of Chemistry, the Division of Molecular and Cellular Biosciences, and the Division of Biological Instrumentation and Resources, addresses two hypotheses. First, that a highly orientationally ordered, functional protein film can be self-organized by adsorption at a highly ordered liquid/solid interface and that the orientational order of this film can be controlled by changing the nature of this interface, and second, that biochemical function in a protein film is dependent on macroscopic orientational order. The objective of this study is to provide a more detailed understanding of protein films for applications in biosensing and bioseparations devices. During the tenure of this thirty-four month continuing grant, Professor Saavedra, a chemist, and Dr. Li, an optical scientist, will cooperatively apply polarized reflection spectroscopies, namely planar integrated optical waveguide-attenuated total reflection (IOW-ATR) and total internal reflection fluorescence (TIRF), to the in situ characterization of the structure and function of cytochrome c and myoglobin films at the solid-liquid interface. This research addresses both the preparation of ordered, functional protein films and the development of analytical techniques appropriate to characterize these films. The successful development of a general methodology to self-organize proteins into oriented two-dimensional arrays would be highly useful for biotechnological applications. Also, this research should give rise to a clearer understanding of relationships between the structure of hydrated protein films and their macroscopic properties which should aid in protein film design and applications.

该项目由化学部、分子和细胞生物科学部以及生物仪器和资源部共同支持，提出了两个假设。首先，高度定向有序的功能蛋白质膜可以通过在高度有序的液/固界面上的吸附而自组织，并且可以通过改变该界面的性质来控制该膜的取向顺序；其次，蛋白质膜中的生化功能依赖于宏观取向顺序。这项研究的目的是为了更详细地了解蛋白质膜在生物传感和生物分离设备中的应用。在这项为期34个月的持续资助期间，化学家Saavedra教授和光学科学家Li博士将合作应用偏振反射光谱仪，即平面集成光波导衰减全反射(IOW-ATR)和全内反射荧光(TIRF)，原位表征细胞色素c和肌红蛋白在固-液界面的结构和功能。这项研究既涉及有序的功能性蛋白质膜的制备，也涉及适合于表征这些膜的分析技术的发展。成功地开发出一种通用的方法，将蛋白质自组织成定向的二维阵列，将对生物技术应用非常有用。此外，这项研究还将使人们更清楚地了解水合蛋白质膜的结构与其宏观性质之间的关系，这将有助于蛋白质膜的设计和应用。