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Orientation and Function in Self-Organized Protein Films

自组织蛋白质膜的取向和功能

基本信息

批准号：
9726132
负责人：
Scott Saavedra
金额：
$ 41.9万
依托单位：
University of Arizona
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1998
资助国家：
美国
起止时间：
1998-02-01 至 2002-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9726132&HistoricalAwards=false
关键词：
Orientation Function Self Organized Protein

项目摘要

This project, supported in the Analytical and Surface Chemistry Program, focusses on the use of a new method for probing the structure and orientation of protein films formed on well characterized surfaces. Professor Saavedra and his colleagues at the University of Arizona will use integrated optical waveguide- attenuated total reflection and total internal refection fluorescence techniques to characterize heme protein films prepared on Langmuir-Blodgett films and self-assembled polyion films. The focus will be to develop correlations between heme protein orientation, functional properties, and direct electron reactions. Long-term objectives of this work involve use of these protein films in molecular device technologies such as bioanalytical sensing, energy storage/conversion, and bioseparations. A new method using integrated optical waveguides has been developed for studying the structure of heme proteins on surfaces. Professor Saavedra and his colleagues at the University of Arizona developed this very sensitive method to characterize thin protein films formed on well characterized surfaces. These proteins form films on these surfaces through controlled electrostatic interactions. The properties of these thin protein films are projected to have uses in areas such as bioanalytical sensing, energy storage/conversion, and biospecific separations. The development of new molecular devices for applications in several biotechnology areas is the long-term goal of this work.

这个项目得到了分析和表面化学计划的支持，重点是使用一种新的方法来探测在特性良好的表面上形成的蛋白质膜的结构和取向。亚利桑那大学的Saavedra教授和他的同事将使用集成光波导衰减全反射和全内反射荧光技术来表征在Langmuir-Blodgett膜和自组装聚离子膜上制备的血红素蛋白薄膜。重点将是发展血红素蛋白取向、功能特性和直接电子反应之间的相关性。这项工作的长期目标包括在生物分析传感、能量存储/转换和生物分离等分子设备技术中使用这些蛋白质膜。发展了一种利用集成光波导来研究血红素蛋白表面结构的新方法。亚利桑那大学的Saavedra教授和他的同事开发了这种非常敏感的方法来表征在特性良好的表面上形成的薄蛋白质膜。这些蛋白质通过受控的静电相互作用在这些表面形成薄膜。这些蛋白质薄膜的特性预计将在生物分析传感、能量存储/转换和生物特异性分离等领域得到应用。开发应用于多个生物技术领域的新分子器件是这项工作的长期目标。