Synthetic Lethal Mutation Analysis of Yeast CP1

酵母 CP1 的合成致死突变分析

• 批准号: 9406050
• 负责人: Richard Baker
• 金额: $ 30万
• 依托单位: University of Massachusetts Medical School
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9406050&HistoricalAwards=false
• 关键词: Synthetic; Lethal; Mutation; Analysis; Yeast

9406050 Baker CP1 (encoded by the gene CEP1) is a moderately abundant, sequence-specific DNA-binding protein of S. cerevisiae. A member of the general regulatory factor family of yeast chromosomal proteins, CP1 is multifunctional; it performs roles in chromosome segregation and in regulating gene expression. It is believed that CP1 acts primarily as a local organizer of chromatin structure, configuring the DNA into conformations conducive to the assembly of kinetochores and active transcription complexes. To learn more about CP1 action, a synthetic lethal genetic analysis of CP1 is being undertaken. Mutations synthetically lethal with cep1 will be isolated and complementation groups defined. The cep1 synthetic lethal mutations (csl's) will be classified by phenotype; the mutants will be tested for defects in mitotic and meiotic chromosome segregation, defects in transcription, synergistic interaction between the csl and a cis-acting centromere mutation, perturbations in cell cycle progression, and ability to be rescued by partially-active mutant alleles of cep1. Wild-type genes corresponding to the most interesting csl alleles will be isolated from yeast genomic plasmid libraries and characterized. %%% The long term goal of this research is to provide general insights into the biochemical mechanisms that establish eukaryotic chromosome structure and effect changes in that structure to meet functional needs. Chromosome structure is an essential element in both the regulation of gene expression and the proper distribution of chromosomes at cell division. ***

9406050 Baker CP 1（由基因CEP 1编码）是一种中等丰度的、序列特异性的S.啤酒。 CP 1是酵母染色体蛋白的一般调节因子家族的成员，是多功能的;它在染色体分离和调节基因表达中发挥作用。 据信，CP 1主要作为染色质结构的局部组织者，将DNA配置成有利于着丝粒和活性转录复合物组装的构象。 为了更多地了解CP 1的作用，目前正在对CP 1进行合成致死基因分析。 与cep 1合成致死的突变将被分离并定义互补组。 cep 1合成致死突变（csl's）将按表型分类;突变体将检测有丝分裂和减数分裂染色体分离缺陷、转录缺陷、csl和顺式作用着丝粒突变之间的协同作用、细胞周期进程扰动以及被cep 1部分活性突变等位基因拯救的能力。 将从酵母基因组质粒库中分离对应于最有趣的csl等位基因的野生型基因并进行表征。 这项研究的长期目标是提供建立真核细胞染色体结构的生化机制的一般见解，并影响该结构的变化，以满足功能需求。 染色体结构是调控基因表达和细胞分裂时染色体正确分布的基本要素。 ***