Acquisition of Automated DNA Sequencer for Primate Molecular Evolutionary Research

获取用于灵长类分子进化研究的自动 DNA 测序仪

• 批准号: 9414016
• 负责人: Maryellen Ruvolo
• 金额: $ 13.83万
• 依托单位: Harvard University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9414016&HistoricalAwards=false
• 关键词: Acquisition; Automated; DNA; Sequencer; Primate

9414016 Ruvolo With National Science Foundation support, Dr. Maryellen Ruvolo will purchase an automated DNA sequencer system consisting of: 1. an Applied Biosystems automated DNA sequencer with the necessary software for editing and analyzing newly-generated sequence files and for DNA microsatellite typing; and 2. a DNA data storage system consisting of: an external hard disk drive for ready retrieval and manipulation of DNA sequence files and a magneto- optical drive for long-term, high stability storage of DNA sequence files. The research of Dr. Ruvolo and her colleagues uses DNA sequence data and the purchase of this instrumentation will exponentially increase the laboratory's productivity. Research on primate systematics and evolution constitutes the focus of the laboratory's work and the team is currently involved in a number of projects. These include a broad scale approach to the molecular systematics of primates and a complementary effort on the molecular evolution of the Strepsirhines. The group has begun work to determine the genetic diversity present within extant chimpanzees and is examining mitochondrial DNA sequence and nuclear microsatellite marker variability in East African chimpanzee populations. These chimpanzees are important because of their endangered status as well as the extensive behavioral information available on selected groups. The new instrumentation will also allow the team to expand its examination of hominoid nuclear gene variation and genomic structure. Dr. Ruvolo's laboratory trains undergraduate and graduate students as well as post-doctoral fellows; automated sequencer equipment will assist in this, as well as the research, function.

小行星9414016 在国家科学基金会的支持下，Maryellen Ruvolo博士将购买一个自动DNA测序仪系统，包括：1。 应用生物系统自动DNA测序仪，其具有用于编辑和分析新生成的序列文件以及用于DNA微卫星分型的必要软件;以及2.一种DNA数据存储系统，包括：一个外部硬盘驱动器，用于随时检索和操作DNA序列文件;以及一个磁光盘驱动器，用于长期、高稳定性存储DNA序列文件。Ruvolo博士和她的同事的研究使用DNA序列数据，购买这种仪器将成倍提高实验室的生产力。 灵长类系统学和进化研究是实验室的工作重点，该小组目前参与了一些项目。其中包括一个广泛的方法，灵长类动物的分子系统学和补充努力的分子进化的Strepsirhines。该小组已开始确定现存黑猩猩的遗传多样性，并正在研究东非黑猩猩种群的线粒体DNA序列和核微卫星标记变异性。这些黑猩猩之所以重要，是因为它们的濒危状态以及有关选定群体的广泛行为信息。新的仪器还将使研究小组能够扩大对类人猿核基因变异和基因组结构的研究。 Ruvolo博士的实验室培养本科生和研究生以及博士后研究员;自动测序仪设备将有助于这一点，以及研究，功能。