Acquisition of a Liquid Chromatography Mass Spectrometry System (LC-MS) at Lehman College

这一ARI(学术研究基础设施)应用程序要求为雷曼学院的化学系和生物系配备高压液相色谱-质谱仪(HPLC-MS)系统。该系统将为三名教员的研究以及生物系一名新教员的研究提供便利。雷曼学院有资格成为少数族裔机构。高效液相色谱质谱联用技术是一种分析天然产物的新技术。在一个步骤中，它允许研究人员检测、分离和执行混合物中物质的初步结构表征。由于研究中的混合物可能有数百种成分，因此可以在一次实验中轻松分析数百种化合物。该系统的高压液相色谱(HPLC)组件是已知的分离小分子和大分子的最强大的方法。天然物质的每个细胞中都有数千个这样的分子。在每一次高效液相色谱运行中，都可以看到数百个峰。然而，这些峰不容易在简单的高效液相色谱仪中被识别。在简单的高效液相色谱仪中，化合物的鉴定通常取决于从其他来源获得参比化合物，并证明参比化合物在几种溶剂体系中具有与所述高效液相色谱峰相同的迁移率和紫外光谱。在天然混合物中看到的大多数高效液相色谱峰仍然没有被识别，因为凭直觉和偶然的机会很难找到合适的参考化合物。该仪器的质谱仪(MS)组件从高效液相色谱中提取每个流出峰，将其分解成离子，并确定每个产生的离子的质量。通常可以根据每个高效液相色谱峰的质量和碎裂模式来确定每个高效液相流出物的暂定结构。有了试探性鉴定，通过使用参考化合物或通过计算机搜索MS碎片图案库来确定阳性鉴定要简单得多。

This ARI (Academic Research Infrastructure) Application requests a high pressure liquid chromatography-mass spectrometer (HPLC-MS) system for the Chemistry and Biology departments at Lehman College. This system will facilitate the research of three faculty members, as well as the research of a new faculty member in the Biology Department. Lehman College qualifies as a Minority Institution. HPLC-MS is a new and powerful technique for the analysis of natural products. In one step, it allows researchers to detect, separate, and perform a preliminary structural characterization of substances in a mixture. Since the mixtures under study can have hundreds of components, it allows easy analysis of hundreds of compounds in one experiment. The high pressure liquid chromatography (HPLC) component of the system is the most powerful method known to separate small and large organic molecules. Natural materials have thousands of such molecules in each cell. In each HPLC run, hundreds of peaks can be seen. However, these peaks cannot easily be identified in a simple HPLC apparatus. Compound identification in a simple HPLC apparatus depends typically on the availability of a reference compound from other sources and demonstrating that the reference compound has the same mobility and UV spectrum in several solvent systems as does the HPLC peak in question. Most HPLC peaks seen in natural mixtures remain unidentified since it is not easy to find, by intuition and chance, the appropriate reference compounds. The Mass Spectrometer (MS) component of the instrument takes each effluent peak from the HPLC, fragments it into ions, and determines the mass of each resulting ion. A tentative structural identification for each HPLC effluent compound can usually be determined from the masses and fragmentation patterns of each HPLC peak. With a tentative identification in hand, determination of a positive identification by use of reference compounds or by computer searching o f MS fragmentation pattern libraries is much simpler.