Acquisition of a Nuclear Magnetic Resonance Spectrometer

9414192 购买核磁共振光谱仪 普林斯顿大学化学系要求从化学研究仪器和设施计划中获得资金，用于购买核磁共振（NMR）光谱仪。 目前，普林斯顿大学唯一的高场核磁共振光谱仪是一台使用了七年的JEOL GSX-500。 该光谱仪是以下涉及化学教师及其小组的项目的关键限制因素。 * 沃伦·S沃伦 最近的实验表明，在溶液中蛋白质和大体积水之间的分子间相干转移为我们提供了各种新的应用，以增强生物分子NMR和成像中的信号。 现代光谱仪上的600 MHz数据具有高灵敏度探头和快速梯度脉冲（合作完成），证明了该技术的重要性。 500兆赫数据与现有的光谱仪基本上是无用的这些研究。 * 丹·卡恩 多维NMR广泛用于一个主要项目，旨在了解DNA-碳水化合物的相互作用。 在这些系统中，500 MHz频谱显示出严重的频谱重叠，这在600 MHz频谱中已被证明大大减少。 * 迈克尔·赫克特 系统设计和合成新的蛋白质，进行结构和性质的比较研究，依赖于多核、多维高场NMR的完整归属和溶液结构的确定。 600兆赫的研究已经开始在合作工作。 * John T.格罗夫斯 极高灵敏度的质子核磁共振需要溶解在磷脂膜中的小分子的结构研究，跨膜配体交换的动力学研究。 目前的500兆赫对于这项工作的大部分工作来说不够敏感，这项工作是研究细胞膜功能和动力学的广泛计划的一部分。 * 珍妮特·凯里 蛋白质折叠和稳定性的研究以及这些性质与配体结合活性的关系需要利用现代600 MHz光谱仪提供的分散的多维NMR能力。 这些研究领域跨越生物结构和物理化学，提出了一套非常多样化的要求，只有600 MHz的光谱仪才能满足。 此外，增加第二个高场将为现有500兆赫频谱仪的限制较少的利用提供必要的能力。

9414192 Purchase of a Nuclear Magnetic Resonance Spectrometer The Department of Chemistry at Princeton University requests funds from the Chemistry Research Instrumentation and Facilities program for the purchase of a nuclear magnetic resonance (NMR) spectrometer. Currently, the only high field NMR spectrometer at Princeton University is a seven year old JEOL GSX-500. This spectrometer is the critical limiting factor to the following projects involving Chemistry faculty members and their groups. * Warren S. Warren Recent demonstrations of intermolecular coherence transfer between proteins and bulk water in solution open us a variety of novel applications to enhance signals in biomolecular NMR and imaging. 600 MHz data on a modern spectrometer with high sensitivity probes and fast gradient pulses (done in collaboration) have proven the importance of the technique. 500 MHz data with the existing spectrometer are essentially useless for these studies. * Dan Kahne Multi-dimensional NMR is used extensively in a major project directed towards understanding DNA-carbohydrate interactions. 500 Mhz spectra show severe spectra overlap in these systems, which have been shown to be substantially reduced in 600 MHz spectra. * Michael Hecht Systematic design and synthesis of novel proteins for compartive studies of structure and properties depend on multi-nuclest, multi-dimensional high-field NMR for complete assignment and solution structure determination. 600 Mhz studies have been started in collaborative work. * John T. Groves Extremely high sensitivity proton NMR is required for structural investigations of small molecules dissolved in phospholipid membranes, and for studies of kinetics of transmembrane ligand exchange. The current 500 Mhz is not sufficiently sensitive for much of this work, which is part of a broad program to study the function and dynamics of cellular membranes. * Jannette Carey Studies of protein folding and s tabilty and the relation of these properties to ligand-binding activity require access to multi-dimensional NMR capabilties with the dispersion afforded by a modern 600 MHz spectrometer. These research areas, spanning biostructural and physical chemistry, present an extremely diverse set of requirements that can only be met by a 600 MHz spectrometer. Moreover, the addition of a second high field would provide necessary capacity for less restrictive utilization of the existing 500 MHz spectrometer.