Acquisition of a Protein Sequencer

获得蛋白质测序仪

• 批准号: 9419704
• 负责人: Paul Price
• 金额: $ 10.21万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-01-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9419704&HistoricalAwards=false
• 关键词: Acquisition; Protein; Sequencer

This proposal requests funds for a replacement protein sequencer for the UCSD Biology Department. The existing sequencer has operated for 10 years and has analyzed more than 1650 samples for 130 laboratories. However, existing sequencer is obsolete and the day is rapidly approaching when it will no longer be repairable. In addition, improvements in sequencer technology and chemistry have been made since the purchase of the existing sequencer. Cycle times have been shortened by 50%, reagent consumption has been reduced, sensitivity has been improved from 100 pmole to less than 2 pmole of sample, and the introduction of multiple cartridges offers significantly increased sample throughput. The use of the protein sequence information includes the necessary first step in the construction of oligonucleotide probes and eventual cloning of genes, investigation of the posttranslational modifications in proteins which are important for protein function, identification of functionally important regions of proteins after amino acid modifications and peptide isolation, investigations of evolution in genes from various species, and the identification of proteins as novel, new proteins by comparing N-terminal sequence data with the available protein sequence data bases.

该提案要求为UCSD生物系更换蛋白质测序仪提供资金。 现有的测序仪已经运行了10年，为130个实验室分析了1650多个样品。 然而，现有的测序仪是过时的，这一天正在迅速接近时，它将不再是可修复的。 此外，自购买现有测序仪以来，测序仪技术和化学方面已经有所改进。 循环时间缩短了50%，试剂消耗减少了，灵敏度从100 pmole提高到小于2 pmole的样品，多个检测盒的引入显著提高了样品通量。 蛋白质序列信息的使用包括构建寡核苷酸探针和最终克隆基因的必要的第一步，研究蛋白质中对蛋白质功能重要的翻译后修饰，鉴定氨基酸修饰和肽分离后蛋白质的功能重要区域，研究来自不同物种的基因进化，以及通过将N-末端序列数据与可用的蛋白质序列数据库进行比较来鉴定蛋白质为新颖的新蛋白质。