Cold Spring Harbor Laboratory Course on the Early Development of Xenopus

近年来，由于许多新方法应用于经典问题，发育生物学领域取得了巨大进展，因此需要开设高级课程来培养新的研究人员来研究那些动物模型，如非洲爪蟾，这些动物模型在该领域的新发现中起着重要作用。目前正在寻求资金继续开展冷泉港实验室关于非洲爪蟾早期发育的课程，这门课程作为冷泉港现代生物学若干领域的综合课程的一部分已经教授了三年。本课程提供了广泛的实验室接触生物学和胚胎的操作从非洲爪蟾青蛙。爪蟾是一种特别适合研究早期发育的脊椎动物，因为从受精开始，可以很容易地获得大量的胚胎。胚胎发育迅速，胚胎的大尺寸有利于对其进行显微操作，以便分析在母体胚胎中不可行的胚胎诱导等重要问题。本课程既适合那些具有分子生物学和发育生物学知识，但没有爪蟾经验的人，也适合那些有一些爪蟾经验的人，他们希望增加他们的技术技能。将涉及的具体领域如下：1。照顾和处理成年非洲爪蟾。诱导排卵，收集卵子，分离睾丸和受精。2. 胚胎发育和解剖的阶段。3. 全载原位杂交和免疫组织化学。4. 带mRNA和DNA构建体的卵和卵母细胞显微注射。5. 胚胎的显微操作，包括诱导和移植试验。6. 细胞周期提取物的制备和应用。预计在拟议的支助期间，课程的基本组织和大部分课程材料将保持不变，但随着实地工作的进展，将讨论新的问题并将其纳入实验室。：

9513994 Grodzicker Dramatic progress in the field of developmental biology in recent years has resulted from the application of a number of new approaches to classical problems, and led to the need for advanced courses to train new investigators to study those animal models, such as Xenopus, that have been important in making new discoveries in this field. Funds are being sought to continue the Cold Spring Harbor Laboratory course on Early Development of Xenopus laevis, which has now been taught for three years as part of an integrated set of courses at Cold Spring Harbor in a number of areas of modern biology. This course provides extensive laboratory exposure to the biology and manipulation of embryos from the frog Xenopus laevis. Xenopus is a vertebrate uniquely suited for studies of early development, since large numbers of embryos, from fertilization onward, can easily be obtained. Development is rapid and the large size of the embryos facilitates their micromanipulation to allow analysis of such important issues as embryonic induction that are not feasible in mama embryos. The course is suited both for those who have a knowledge of molecular biology and developmental biology, but have had no experience with Xenopus, as well as those with some Xenopus experience who wish to increase their repertoire of techniques. The specific areas that will be covered are listed below: 1. Care and handling of Xenopus adults. Induction of ovulation, egg collection, testis isolation, and fertilization. 2. Stages of embryonic development and anatomy. 3. Whole mount in situ hybridization and immunohistochemistry. 4. Microinjection of eggs and oocytes with mRNA and DNA constructs. 5. Micromanipulation of embryos, including induction and transplantation assays. 6. Preparation and use of cell cycle extracts. It is anticipated that the basic organization of the course and much of the course material will remain unchanged during the proposed period of support, though new issues will be discussed and integrated into the laboratory as the field progresses. :