Cold Spring Harbor Laboratory Course on the Early Development of Xenopus

冷泉港非洲爪蟾早期发育实验室课程

• 批准号: 9513994
• 负责人: Terri Grodzicker
• 金额: $ 3万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9513994&HistoricalAwards=false
• 关键词: Cold; Spring; Harbor; Laboratory; Course

9513994 Grodzicker Dramatic progress in the field of developmental biology in recent years has resulted from the application of a number of new approaches to classical problems, and led to the need for advanced courses to train new investigators to study those animal models, such as Xenopus, that have been important in making new discoveries in this field. Funds are being sought to continue the Cold Spring Harbor Laboratory course on Early Development of Xenopus laevis, which has now been taught for three years as part of an integrated set of courses at Cold Spring Harbor in a number of areas of modern biology. This course provides extensive laboratory exposure to the biology and manipulation of embryos from the frog Xenopus laevis. Xenopus is a vertebrate uniquely suited for studies of early development, since large numbers of embryos, from fertilization onward, can easily be obtained. Development is rapid and the large size of the embryos facilitates their micromanipulation to allow analysis of such important issues as embryonic induction that are not feasible in mama embryos. The course is suited both for those who have a knowledge of molecular biology and developmental biology, but have had no experience with Xenopus, as well as those with some Xenopus experience who wish to increase their repertoire of techniques. The specific areas that will be covered are listed below: 1. Care and handling of Xenopus adults. Induction of ovulation, egg collection, testis isolation, and fertilization. 2. Stages of embryonic development and anatomy. 3. Whole mount in situ hybridization and immunohistochemistry. 4. Microinjection of eggs and oocytes with mRNA and DNA constructs. 5. Micromanipulation of embryos, including induction and transplantation assays. 6. Preparation and use of cell cycle extracts. It is anticipated that the basic organization of the course and much of the course material will remain unchanged during the proposed period of support, though new issues will be discussed and integrated into the laboratory as the field progresses. :

9513994 Grodzicker 近年来，发育生物学领域的巨大进步源于对经典问题的许多新方法的应用，并导致需要高级课程来培训新的研究人员来研究这些动物模型，例如非洲爪蟾，这些动物模型对于在该领域取得新发现非常重要。正在寻求资金继续冷泉港实验室关于非洲爪蟾早期发育的课程，该课程现已教授三年，作为冷泉港现代生物学许多领域的综合课程的一部分。本课程提供广泛的实验室知识，让您了解非洲爪蟾胚胎的生物学和操作。非洲爪蟾是一种特别适合研究早期发育的脊椎动物，因为从受精开始就可以轻松获得大量胚胎。发育迅速，胚胎的大尺寸有利于它们的显微操作，从而可以分析胚胎诱导等在母体胚胎中不可行的重要问题。该课程既适合那些具有分子生物学和发育生物学知识但没有非洲爪蟾经验的人，也适合那些有一些非洲爪蟾经验但希望增加技术储备的人。所涵盖的具体领域如下所列： 1. 非洲爪蟾成虫的护理和处理。诱导排卵、收集卵子、分离睾丸和受精。 2. 胚胎发育和解剖阶段。 3.整体原位杂交和免疫组化。 4. 用 mRNA 和 DNA 构建体显微注射卵子和卵母细胞。 5. 胚胎显微操作，包括诱导和移植测定。 6.细胞周期提取物的制备和使用。 预计课程的基本组织和大部分课程材料将在拟议的支持期内保持不变，但随着该领域的进展，新问题将被讨论并整合到实验室中。 :