Integrative genomic and genetic analysis of nonhost resistance across Triticeae species (TritNONHOST)

该项目的科学目的是确定和探索小麦和大麦对三种具有巨大经济和生态影响的病原体（锈菌属）的非宿主抗性的遗传框架，白粉病（Blumeria spp.）和稻瘟病菌（Magnaporthe spp.）谷物中的病害一个全球性的，差异基因表达分析，比较兼容的主机和nonhost的相互作用，将允许识别共同的，植物物种特异性和病原体speciespathways参与小麦和大麦的非宿主抗性。功能基因组学方法将被用来测试潜在的非寄主抗性基因在相应的谷类物种的非适应性以及适应病原体的抗性的功能。功能数据将与非寄主抗性分离的新群体的定量遗传数据相结合，以便为候选基因在小麦族非寄主抗性中的作用提供一致的证据（图1）。该项目的成果将使我们对谷物非宿主抗性生物学的基本内部运作有一个新的认识，这将为欧洲植物育种业提供实现对主要病原体的可持续抗性所需的知识和工具。最终目的是将非寄主抗性的遗传成分从一种谷物转移到另一种谷物，以实现对适应病原体的“非寄主样”抗性。

The scientific aim of this project is to identify and explore the genetic framework for nonhost resistance in wheat and barley to three pathogens of enormous economic and ecological impact causing rust (Puccinia spp.), powdery mildew (Blumeria spp.) and blast (Magnaporthe spp.) disease in cereals. A global, differential gene-expression analysis, comparing compatible host and nonhost interactions will allow the identification of common, plant species-specific and pathogen speciesspecific pathways involved in nonhost resistance in wheat and barley. Functional genomics approaches will be used to test the function of potential nonhost resistance genes in the corresponding cereal species for resistance to non-adapted as well as adapted pathogens. The functional data will be combined with quantitative genetic data from novel populations segregating for nonhost resistance, in order to provide convergent evidence for a role of candidate genes in nonhost resistance of Triticeae (Figure 1). The outputs from this project will provide us with a new level of understanding of the fundamental inner workings of the biology of nonhost resistance in cereals that will provide the European plant breeding industry with the knowledge and tools required to achieve sustainable resistance to major pathogens. The ultimate aim is to transfer the genetic components of nonhost resistance from one cereal to the other to achieve “nonhost-like” resistance to the adapted pathogen.