CAREER: Nitrite and Sulfite Reduction by Myoglobin in Surfactant Films

无机化学、生物无机化学和金属有机化学项目的这一职业奖支持加州大学欧文分校的帕特里克·J·法默的研究和教育计划。这项拟议研究的目标是了解包含在电极上的膜中的肌红蛋白(Mb)的新的仿生化学。这种Mb-膜具有电催化亚硝酸盐和亚硫酸盐还原酶活性。通过使用修饰的肌红蛋白，还原酶的功能活性结构模型将用电化学方法进行研究。研究的目的包括鉴定导致N-N和S-S产物的中间体的性质，并探索还原的铁结合的N-和S-氧化物中间体的氧原子转移反应。薄膜中修饰的肌红蛋白将被用作亚硝酸盐和亚硫酸盐还原酶的模型。将针对对健康相关职业感兴趣的学生开发一门本科课程，并为研究生设立一项培训计划，涉及合作研究、研究实验室轮换和研讨会。

This CAREER Award in the Inorganic, Bioinorganic and Organometallic Chemistry Program supports the research and education plans of Patrick J. Farmer at the University of California at Irvine. The goal of the proposed research is to understand new biomimetic chemistry of myoglobin (Mb) contained within a film on an electrode. Such Mb-films have electrocatalytic nitrite and sulfite reductase activity. By using modified myoglobins, functionally active structural models for the reductases will be studied by electrochemical methods. Aims of the research include identifying the nature of intermediates leading to N-N and S-S products and to probe oxygen atom transfer reactions from reduced Fe-bound N- and S-oxide intermediates. Modified myoglobins in thin films will be used as models of nitrite and sulfite reductase enzymes. An undergraduate course aimed at students interested in health-related careers will be developed and a training program for graduate students instituted involving collaborative research, rotations in research laboratories and seminars.