Development of a Multichannel Time-Resolved Fluorometer for Macro and Mictrostudies

本项目"用于宏观和微观研究的多通道时间分辨荧光计的开发“旨在更新和提高时间分辨荧光计的能力， 西奈山的荧光设施，提供有关生物大分子及其复合物的结构和动力学的相关信息。该设施目前的仪器部分由1986年国家科学基金会题为“用于时间分辨成像的多用户激光器”的奖金（DMB-8516318）资助。新提案有三个主要目标： 用最先进的脉冲激光系统取代过时的、有限波长范围的脉冲激光系统， 激光系统，能够提供紫外和可见光激发波长所需的许多 研究项目 建造和实施一个新的样品室，同时提供三个检测通道，以提高 时间分辨荧光强度和各向异性数据集的收集和精度 联合收割机将时间分辨荧光与显微镜相结合，以允许对“微量”样品进行研究 概述了一个为期三年的计划，主要组件的大部分收购/开发将在第一年进行。实施、测试和试运行将在接下来的两年内进行。

This project, `Development of a Multichannel Time-Resolved Fluorometer for Macro and Microstudies', is to update and improve the ability of the Time- Resolved Fluorescence Facility at Mount Sinai to provide pertinent information about the structure and dynamics of biological macromolecules and their complexes. The present instrumentation in the Facility was funded in part by a 1986 NSF award (DMB-8516318) entitled `A Multi-User Laser for Time-Resolved Fluorescenc'. The new proposal has three main objectives: replacement of the outdated, limited-wavelength range pulsed laser system with a state-of-the-art pulsed laser system capable of providing the UV and visible excitation wavelengths required by numerous research programs construct and implement a new sample chamber with three simultaneous detection channels to enhance the collection and the precision of time-resolved fluorescence intensity and anisotropy data sets combine time-resolved fluorescence with microscopy to permit studies on 'micro' samples A three year program is outlined, with most of the acquisition/development of the major components to occur in the first year. Implementation, testing, and commissioning will be performed in the following two years.