Monitoring Chip Based Electrophoretic Separations with Surface Enhanced Raman Spectroscopy

使用表面增强拉曼光谱监测基于芯片的电泳分离

• 批准号: 273006689
• 负责人: Professor Dr. Detlev Belder
• 金额: --
• 依托单位: Institut für Analytische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/273006689?language=en
• 关键词: Monitoring; Chip; Based; Electrophoretic; Separations

The potential of surface enhanced Raman Spectroscopy is investigated as a label free detection technique in chip electrophoresis. The aim of the project is the development and of methods and instrumental approaches to realize and study surface enhanced Raman detection in chip-based separation devices. For this purpose two different approaches to make use of the SERS effect in microfluidic chips will be investigated to monitor tiny amounts of electrophoretically separated analytes at high speed and sensitivity. While in one part of the studies the integration of stationary SERS-targets will be investigated the other approach is focused on the use of colloidal solutions of SERS active nanoparticles. The simultaneously acquired data on electrophoretic mobility and Raman spectra shall be utilized to unequivocally identify compounds in complex mixtures. A primary aim the research project is to cast light on the potential of SERS-Detection in microfluidic separation techniques in the context of alternative established methods like fluorescence detection or mass spectrometry.

研究了表面增强拉曼光谱作为芯片电泳中的无标记检测技术的潜力。该项目的目的是开发方法和仪器方法来实现和研究基于芯片的分离装置中的表面增强拉曼检测。 为此，将研究在微流控芯片中利用 SERS 效应的两种不同方法，以高速和灵敏地监测微量的电泳分离分析物。 虽然部分研究将研究固定 SERS 靶标的整合，但另一种方法则侧重于使用 SERS 活性纳米粒子的胶体溶液。同时获取的电泳迁移率和拉曼光谱数据应用于明确识别复杂混合物中的化合物。该研究项目的主要目的是在荧光检测或质谱等替代既定方法的背景下，揭示 SERS 检测在微流体分离技术中的潜力。

项目成果

Surface enhanced Raman spectroscopy in microchip electrophoresis.

微芯片电泳中的表面增强拉曼光谱

• DOI: 10.1016/j.chroma.2018.02.014
• 发表时间: 2018
• 期刊: Journal of chromatography. A
• 作者: A. Tycova;R. F. Gerhardt;D. Belder
• 通讯作者: D. Belder

Microchip HPLC separations monitored simultaneously by coherent anti-Stokes Raman scattering and fluorescence detection

• DOI: 10.1007/s00604-016-2012-3
• 发表时间: 2016
• 期刊: Microchimica Acta
• 影响因子: 5.7
• 作者: D. Geissler;Josef J. Heiland;Carsten Lotter;D. Belder

Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water

• DOI: 10.1002/elps.202000048
• 发表时间: 2020-05
• 期刊: ELECTROPHORESIS
• 影响因子: 2.9
• 作者: Benjamin Krafft;Anna Týcová;Raphael D Urban;Christian Dusny;D. Belder

On-chip integration of normal phase high-performance liquid chromatography and droplet microfluidics introducing ethylene glycol as polar continuous phase for the compartmentalization of n-heptane eluents.

正相高效液相色谱和液滴微流体的芯片集成，引入乙二醇作为极性连续相，用于正庚烷洗脱液的区室化

• DOI: 10.1016/j.chroma.2019.460653
• 发表时间: 2020
• 期刊: Journal of chromatography. A
• 作者: A. J. Peretzki;D. Belder
• 通讯作者: D. Belder

A microfluidic device enabling surface-enhanced Raman spectroscopy at chip-integrated multifunctional nanoporous membranes

• DOI: 10.1007/s00216-019-02228-9
• 发表时间: 2019-12-03
• 期刊: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
• 影响因子: 4.3
• 作者: Krafft, Benjamin;Panneerselvam, Rajapandiyan;Belder, Detlev
• 通讯作者: Belder, Detlev