Monitoring Chip Based Electrophoretic Separations with Surface Enhanced Raman Spectroscopy

研究了表面增强拉曼光谱在芯片电泳中作为一种无标记检测技术的潜力。该项目的目的是开发和研究在芯片分离设备中实现和研究表面增强拉曼检测的方法和仪器途径。为此，将研究两种不同的方法来利用微流控芯片中的SERS效应，以高速和灵敏地监测微量的电泳分离分析物。在研究的一部分中，将研究固定的SERS靶标的集成，另一种方法侧重于使用SERS活性纳米颗粒的胶体溶液。应利用同时获得的关于电泳迁移率和拉曼光谱的数据来明确地识别复杂混合物中的化合物。该研究项目的一个主要目的是揭示SERS检测在微流控分离技术中的潜力，以替代现有的方法，如荧光检测或质谱分析。

The potential of surface enhanced Raman Spectroscopy is investigated as a label free detection technique in chip electrophoresis. The aim of the project is the development and of methods and instrumental approaches to realize and study surface enhanced Raman detection in chip-based separation devices. For this purpose two different approaches to make use of the SERS effect in microfluidic chips will be investigated to monitor tiny amounts of electrophoretically separated analytes at high speed and sensitivity. While in one part of the studies the integration of stationary SERS-targets will be investigated the other approach is focused on the use of colloidal solutions of SERS active nanoparticles. The simultaneously acquired data on electrophoretic mobility and Raman spectra shall be utilized to unequivocally identify compounds in complex mixtures. A primary aim the research project is to cast light on the potential of SERS-Detection in microfluidic separation techniques in the context of alternative established methods like fluorescence detection or mass spectrometry.