Interaction Between Substratum Surface Chemistry, Conformation of Contaminant Upon Adsorption, and Availability for Bacterial Degradation

基质表面化学、吸附后污染物构象和细菌降解可用性之间的相互作用

• 批准号: 9729413
• 负责人: James Bryers
• 金额: $ 34.8万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至 2000-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9729413&HistoricalAwards=false
• 关键词: Interaction; Between; Substratum; Surface; Chemistry

9729413 Bryers We hypothesize that the bioavailability of adsorbed organic contaminants is determined by their conformation upon adsorption and that this conformation is affected by the chemical properties of the surface and the contaminant. Research will quantify the surface chemistry effects on adsorption conformation and the subsequent availability of adsorbed contaminants for bacterial degradation. We will determine the degradation kinetics for model compounds separately and as mixtures in suspended pure and mixed bacterial cultures. Biofilm formation kinetics for pure and defined mixed cultures, cultivated on a benign substrate will be determined. Silicon crystals of known texture will serve as control surfaces. Next we will use self assembled monolayers (SAMs) to bind desired functional groups to silicon substrata; thus controlling local surface chemistry at constant topography. Variation in surface chemistry of each substratum will be determined by a regimen of surface analyses. Single organic contaminants, then mixtures of those contaminants will be exposed to the various substrata. Contaminant configuration will vary due to the pre-treatment chemistry or the spatial variation in that treatment. Contaminant conformations will be quantified using the techniques above. Rates of bacterial cell adhesion and biofilm formation will be measured non-invasively using fluorescent confocal microscopic image analysis. Degradation of adsorbed contaminants will be monitored using fluorescent reporter genes. Rates of contaminant degradation, alone or within a mixture, will be correlated to the molecular configuration adsorbed to the surface.

我们假设被吸附的有机污染物的生物利用度是由它们在吸附时的构象决定的，而这种构象受表面和污染物的化学性质的影响。研究将量化表面化学对吸附构象的影响，以及随后吸附的污染物对细菌降解的可用性。我们将分别测定模型化合物的降解动力学，以及作为悬浮纯细菌和混合细菌培养物的混合物。在良性基质上培养的纯混合培养物的生物膜形成动力学将被确定。已知纹理的硅晶体将作为控制表面。接下来，我们将使用自组装单层（sam）将所需的官能团结合到硅基上；从而控制局部表面化学在恒定的地形。每种基质的表面化学变化将通过表面分析方案来确定。单一有机污染物，然后这些污染物的混合物将暴露在各种基质中。污染物的结构会因预处理化学或处理中的空间变化而变化。污染物构象将使用上述技术进行量化。细菌细胞粘附率和生物膜形成将采用荧光共聚焦显微图像分析非侵入性测量。将利用荧光报告基因监测吸附污染物的降解情况。污染物的降解速率，单独或在混合物中，将与吸附在表面的分子构型相关。