Engineering Research Equipment Grant: Attenuated Total Reflectance/Fourier Transform Infrared Spectrometer Purchase

傅里叶变换红外光谱仪 衰减全反射（ATR）能力 购买用于中心内的教师专用 生物化学工程 没有这样的文书， 工程学院 最近的FT-IR光谱仪 位于杜克大学化学系，其用途是 专门用于非生物样本。 主要研究者正在进行的和未来的项目 目前需要ATR/FT-IR光谱仪的包括： 口腔粘膜附着力的原位ATR/FT-IR光谱研究 生物膜形成 1.细菌粘附分子化学 固定化细胞生理学 2.细菌生物膜中的大分子物质传递 3.细菌生物膜脱落的机理 吸附蛋白质膜的ATR/FT-IR光谱研究 1.吸附后蛋白质变性 2.基质的理化性质对土壤肥力的影响 吸附蛋白膜 3.配体与吸附蛋白结合的机制 依赖锚定的哺乳动物细胞的ATR/FT-IR光谱 过程 1.基底预处理对支抗依赖性的影响 哺乳动物细胞粘附 2.剪切应力和生长因子对锚固的影响- 哺乳动物细胞生理学

A FOURIER TRANSFORM INFRARED (FT-IR) Spectrometer with Attenuated Total Reflectance (ATR) capabilities is being purchased for the dedicated use of faculty within the Center for Biochemical Engineering. No such instrument exists within the School of Engineering. The nearest FT-IR spectrometer resides in the Chemistry Department at Duke and its use is dedicated to non-biological samples. On-going and future projects of the principal investigators currently requiring an ATR/FT-IR spectrometer include: In-Situ ATR/FT-IR SPECTROSCOPY OF BACTERIAL ADHESION AND BIOFILM FORMATION 1. Molecular Chemistry of Bacterial Adhesion and Immobilized Cell Physiology 2. Macromolecule Mass Transport Through Bacterial Biofilms 3. Mechanisms Governing Bacterial Biofilm Sloughing ATR/FT-IR SPECTROSCOPY OF ADSORBED PROTEIN FILMS 1. Protein Denaturation Upon Adsorption 2. Effects of Physiochemical Nature of Substratum on Adsorbed Protein Films 3. Mechanisms of Ligand binding to Adsorbed Proteins ATR/FT-IR SPECTROSCOPY OF ANCHORAGE-DEPENDENT MAMMALIAN CELL PROCESSES 1. Substratum Pretreatment Effects on Anchorage-Dependent Mammalian Cell Adhesion 2. Shear Stress and Growth Factor Effects on Anchorage- Dependent Mammalian Cell Physiology