Enhanced Enzyme Productivity in Microorganisms Experiencing Prolonged System Transients

提高经历长时间系统瞬变的微生物的酶生产力

• 批准号: 8711612
• 负责人: James Bryers
• 金额: $ 19.78万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-15 至 1991-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8711612&HistoricalAwards=false
• 关键词: Enhanced; Enzyme; Productivity; Microorganisms; Experiencing

Narrative: Enzymes are proteins contained within cells that serve as biological catalysts of specific biochemical reactions. Production of a myriad of enzymes for both analytical, diagnostic, or therapeutic uses is a major concern of the biochemical industry. Enzymes are produced by cultivating microorganisms under optimum growth conditions, collecting the microbe, and extracting and purifying the desired protein. Currently, two classes of enzymes, alcohol- and glucose-oxidizing enzymes, are produced commercially using the yeast, Hansenula polymorpha, grown in continuous culture using a single carbon energy substrate; either methanol or glucose. Results of past work indicate levels of both enzymes can be doubled or tripled if a mixture of glucose and methanol is used at low growth rate. This research intends to extend the above steady-state work to investigate effects of prescribed shifts in both system growth rate and substrate carbon mixtures on enzymes productivity. Results will increase our understanding of microbial regulatory processes under transient conditions.

叙述：酶是细胞内含有的蛋白质， 特定生化反应的生物催化剂。 生产 用于分析、诊断或 治疗用途是生物化学工业的主要关注点。 酶是通过在最佳条件下培养微生物来生产的。 生长条件，收集微生物，提取， 纯化所需的蛋白质。 目前，两类酶，酒精氧化酶和葡萄糖氧化酶 酶，是商业生产使用酵母，汉逊酵母 polymorpha，使用单一碳能量在连续培养中生长 底物;甲醇或葡萄糖。 过去工作的成果 表明两种酶的水平可以加倍或三倍， 在低生长速率下使用葡萄糖和甲醇的混合物。 本研究旨在将上述稳态工作扩展到 研究两个系统增长率中规定变化的影响 和底物碳混合物对酶生产率的影响。 结果将 增加我们对微生物调控过程的理解， 瞬态条件