Interaction Between Substratum Surface Chemistry, Conformation of Contaminant Upon Adsorption, and Availability for Bacterial Degradation

9817719BRYERS我们假设吸附的有机污染物的生物利用度是由其吸附时的构象决定的，并且该构象受到表面和污染物的化学性质的影响。研究将量化表面化学对吸附构象的影响以及随后吸附污染物用于细菌降解的有效性。我们将分别确定模型化合物的降解动力学以及悬浮纯细菌培养物和混合细菌培养物中的混合物的降解动力学。将确定在良性基质上培养的纯培养物和特定混合培养物的生物膜形成动力学。已知结构的硅晶体将用作控制表面。接下来，我们将使用自组装单层（SAM）将所需的官能团结合到硅基底上；从而在恒定的形貌下控制局部表面化学。每个基质的表面化学变化将通过表面分析方案来确定。单一有机污染物，然后这些污染物的混合物将暴露于各种基质。污染物的配置会因预处理化学成分或处理中的空间变化而变化。将使用上述技术对污染物构象进行定量。将使用荧光共焦显微图像分析非侵入性地测量细菌细胞粘附和生物图像形成的速率。将使用荧光报告基因监测吸附污染物的降解。污染物单独或在混合物中的降解速率将与吸附到表面的分子构型相关。

9817719BRYERSWe hypothesize that the bioavailability of adsorbed organic contaminants is determined by their conformation upon adsorption and that this conformation is affected by the chemical properties of the surface and the contaminant. Research will quantify the surface chemistry effects on adsorption conformation and the subsequent availability of adsorbed contaminants for bacterial degradation.We will determine the degradation kinetics for model compounds separately and as mixtures in suspended pure and mixed bacterial cultures. Biofilm formation kinetics for pure and defiined mixed cultures, cultivated on a benign substrate will be determined. Silicon crystals of known texture will serve as control surfaces.Next we will use self assembled monolayers (SAMs) to bind desired functional groups to silicon sub strata; thus controlling local surface chemistry at constant topography. Variation in surface chemistry of each substratum will be determined by a regimen of surface analyses.Single organic contaminants, then mixtures of those contaminants will be exposed to the various sub strata. Contaminant configuration will vary due to the pre-treatment chemistry or the spatial variation in that treatment. Contaminant conformations will be quantified using the techniques above.Rates of bacterial cell adhesion and bio~im formation will be measured non-invasively using ~uorescent confocal microscopic image analysis. Degradation of adsorbed contarninants will be monitored using ~uorescent reporter genes. Rates of contaminant degradation, alone or within a mixture, will be correlated to the molecular configuration adsorbed to the surface.