Interaction Between Substratum Surface Chemistry, Conformation of Contaminant Upon Adsorption, and Availability for Bacterial Degradation

9817719我们假设被吸附的有机污染物的生物利用度是由它们在吸附时的构象决定的，而这种构象受表面和污染物的化学性质的影响。研究将量化表面化学对吸附构象的影响，以及随后吸附的污染物对细菌降解的可用性。我们将分别测定模型化合物的降解动力学，以及作为悬浮纯细菌和混合细菌培养物的混合物。生物膜形成动力学的纯和定义的混合培养，培养在一个良性的底物将确定。已知纹理的硅晶体将作为控制表面。接下来，我们将使用自组装单层（sam）将所需的官能团结合到硅下层；从而控制局部表面化学在恒定的地形。每种基质的表面化学变化将通过表面分析方案来确定。单一有机污染物，然后这些污染物的混合物将暴露在不同的下层。污染物的结构会因预处理化学或处理中的空间变化而变化。污染物构象将使用上述技术进行量化。细菌细胞的黏附率和生物形成率将使用荧光共聚焦显微图像分析无创测量。利用荧光报告基因监测吸附污染物的降解情况。污染物的降解速率，单独或在混合物中，将与吸附在表面的分子构型相关。

9817719BRYERSWe hypothesize that the bioavailability of adsorbed organic contaminants is determined by their conformation upon adsorption and that this conformation is affected by the chemical properties of the surface and the contaminant. Research will quantify the surface chemistry effects on adsorption conformation and the subsequent availability of adsorbed contaminants for bacterial degradation.We will determine the degradation kinetics for model compounds separately and as mixtures in suspended pure and mixed bacterial cultures. Biofilm formation kinetics for pure and defiined mixed cultures, cultivated on a benign substrate will be determined. Silicon crystals of known texture will serve as control surfaces.Next we will use self assembled monolayers (SAMs) to bind desired functional groups to silicon sub strata; thus controlling local surface chemistry at constant topography. Variation in surface chemistry of each substratum will be determined by a regimen of surface analyses.Single organic contaminants, then mixtures of those contaminants will be exposed to the various sub strata. Contaminant configuration will vary due to the pre-treatment chemistry or the spatial variation in that treatment. Contaminant conformations will be quantified using the techniques above.Rates of bacterial cell adhesion and bio~im formation will be measured non-invasively using ~uorescent confocal microscopic image analysis. Degradation of adsorbed contarninants will be monitored using ~uorescent reporter genes. Rates of contaminant degradation, alone or within a mixture, will be correlated to the molecular configuration adsorbed to the surface.