Use of Gene Therapy to Prevent Bacterial Infections of Biomaterials

利用基因疗法预防生物材料的细菌感染

• 批准号: 9870952
• 负责人: James Bryers
• 金额: $ 38.6万
• 依托单位: University of Connecticut Health Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-15 至 2002-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9870952&HistoricalAwards=false
• 关键词: Use; Gene; Therapy; Prevent; Bacterial

9870952 Bryers The objective of this research is to determine the potential to block bacterial colonization of a biomaterial by biologically interfering with the bacterial adhesion process. In a radical approach, the investigator proposes to deliver from the biomaterial itself, plasmid genes that upon incorporation into both arriving macrophage and fibroblast cells, will express a heterologous polypeptide that blocks bacterial biofilm formation. The research plan has three major goals: (1) the construction of plasmids containing the gene for a antibacterial adhesion peptide, (2) the quanification of plasmid gene transfer in cells via in vitro culture, and (3) the measurement of gene products and their ability to inhibit bacterial film formation. The deterrence of biofilm formation on materials has important uses in a variety of applications. These include food processing and handling equipment, heat exchange devices for use in power and food industries, and biomedical instruments and devices. ***

本研究的目的是确定通过干扰细菌粘附过程来阻止细菌在生物材料上定植的潜力。在一种激进的方法中，研究者提出从生物材料本身传递质粒基因，当质粒基因被结合到到达的巨噬细胞和成纤维细胞中时，会表达一种阻止细菌生物膜形成的异源多肽。该研究计划有三个主要目标：(1)构建含有抗菌粘附肽基因的质粒；(2)通过体外培养定量质粒基因在细胞中的转移；(3)测量基因产物及其抑制细菌膜形成的能力。生物膜在材料上形成的威慑在各种应用中都有重要的用途。这些包括食品加工和处理设备，用于电力和食品工业的热交换设备，以及生物医学仪器和设备。＊＊＊