"An Integrated Approach to Instruction in Electrochemistry and Liquid Chromatography: A LabVIEW-Based Pulsed Amperometric Detection System"

“电化学和液相色谱的综合教学方法：基于 LabVIEW 的脉冲电流检测系统”

• 批准号: 9851571
• 负责人: Mark Jensen
• 金额: $ 0.93万
• 依托单位: Concordia College at Moorhead
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9851571&HistoricalAwards=false
• 关键词: Integrated; Approach; Instruction; Electrochemistry; Liquid

Traditional courses in analytical chemistry typically devote a significant amount of time to the study of both electrochemical methods and analytical separations. While most students have little difficulty understanding and applying the basic principles of separation techniques, the fundamentals of electrochemistry tend to remain much more abstract. Unfortunately, most traditional electrochemistry laboratory exercises do little to make the subject come alive for the student. The laboratory project outlined in this proposal is designed to provide students with a dynamic interactive platform for instruction in electrochemistry and chromatography by focusing on the development of an electrochemical detection scheme for liquid chromatography (LC-EC). A four-week laboratory sequence emphasizing the development of an LC-EC method for carbohydrate determinations will be designed for our senior-level instrumental analysis course. (A one-week exercise based on applications of this method for quality control will be incorporated into our sophomore-level introductory analytical course.) At the heart of this project is a LabVIEW-based data acquisition interface. Each electrochemical experiment will be controlled by a LabVIEW program, commonly referred to as a virtual instrument, which gives students complete control of all electrode potential and timing parameters. After an initial focus on the fundamentals of cyclic voltammetry, the voltammetric behavior of glucose will be applied to the development of a detection scheme for carbohydrates in a flow-through system. Students will be led to discover that a pulsed potential sequence is needed in order to obtain a reproducible response. A multi-step potential waveform (E vs. t) will be developed to include both formation and dissolution of a surface oxide layer, allowing the student to visualize electrochemical processes at the molecular level. This waveform will then be applied to the quantitative determination of carbohydrates separated by anion-exchange li quid chromatography.

分析化学的传统课程通常投入大量时间来研究电化学方法和分析分离。虽然大多数学生在理解和应用分离技术的基本原理上没有什么困难，但电化学的基础知识往往仍然更加抽象。不幸的是，大多数传统的电化学实验室练习对于让学生生动地了解该主题几乎没有什么帮助。该提案中概述的实验室项目旨在通过重点开发液相色谱（LC-EC）电化学检测方案，为学生提供电化学和色谱教学的动态交互平台。我们将为我们的高级仪器分析课程设计为期四周的实验室序列，重点是开发用于碳水化合物测定的 LC-EC 方法。 （基于这种质量控制方法应用的为期一周的练习将纳入我们大二水平的入门分析课程中。）该项目的核心是基于 LabVIEW 的数据采集界面。每个电化学实验都由 LabVIEW 程序（通常称为虚拟仪器）控制，使学生能够完全控制所有电极电位和计时参数。在初步关注循环伏安法的基础知识后，葡萄糖的伏安行为将应用于开发流通系统中碳水化合物的检测方案。将引导学生发现需要脉冲电位序列才能获得可重复的响应。将开发多步电位波形（E 与 t），以包括表面氧化物层的形成和溶解，使学生能够在分子水平上可视化电化学过程。然后将该波形应用于阴离子交换液相色谱分离的碳水化合物的定量测定。