Designer Particles for The Biological Sciences

奖项摘要-9876691该奖项将支持通过光刻和金属沉积制造一种新的金属颗粒，并在生物研究的重要领域对这些颗粒进行测试。这些设计的颗粒将被生产出来，并用于生物化学介导的植物和哺乳动物细胞的遗传转化。将对设计者粒子进行的特殊生物学测试研究包括：(1)利用生物化学方法比较研究黄金设计者粒子与传统钨和金粒子之间的植物遗传转化效率；(2)利用钴设计者粒子研究模型组织的粘弹性响应，方法是使用磁镊子对嵌入模型组织中的磁性设计者粒子施加振荡或静态力；以及(3)比较研究常规金和黄金设计者粒子之间通过生物语言学方法转化哺乳动物细胞的效率。这些研究将包括DNA疫苗的交付，绿色荧光蛋白-肌球蛋白融合蛋白，以及控制视网膜神经元过程的组装和拆卸的分子，突变离子通道。

Award Abstract - 9876691This award will support the fabrication of a new kind of metal particle, by lithography and metal deposition, and the testing of these particles in important areas of biological research. These designer particles will be produced and tested for biolistics-mediated genetic transformation of plant and mammalian cells. The particular biological testing studies to be conducted with the designer particles include (1) a comparative study of plant genetic transformation efficiency by biolistics between the gold designer particles and conventional tungsten and gold particles (2) the use of cobalt designer particles for the study of the viscoelastic response of model tissues by the using magnetic tweezers to apply oscillating or static forces to magnetic designer particles embedded in the model tissue, and (3) a comparative study of mammalian cell transformation efficiency by biolistics between conventional gold particles and gold designer particles. These studies will include the delivery of DNA vaccines, green fluorescent protein-myosin fusion proteins, and molecules controlling assembly and disassembly of neuronal processes in the retina, mutant ion channels.