Experimental Evolutionary Studies of Temperature Adaptation: Evolution in a Constant Thermal Environment

这个项目研究了在恒温条件下生活了数千代的细菌种群的长期进化和遗传变化。采购经理人指数将决定适应这些持续环境的后果，特别是增长的相对适宜性和效率的提高。PI还将检查这些变化是否伴随着在其他热环境中失去功能，即细菌生长和繁殖的温度范围是否下降，以及它们在其他热环境中是否变得比培养它们的环境更不适合和效率更低。PI将对两组细菌谱系进行这些测量，一组在37℃下培养20,000代，另一组在适应其他热环境后在37℃下培养2,000代。这些研究的结果将为预测生物系统对全球气候变化的反应和有关环境适应的一般进化理论提供重要的实验检验。

This project examines long-term evolutionary and genetic changes inpopulations of bacteria living for thousands of generations in constantthermal conditions. The PIs will determine the consequences of adaptation tothese constant environments, specifically, the increase in relative fitnessand efficiency of growth. The PIs will also examine whether these changes areaccompanied by loss of function in other thermal environments, that is,whether the range of temperatures over which the bacteria can grow andreproduce declines and whether they become less fit and less efficient inother thermal environments than that in which they are cultured. The PIs willmake these measurements on two sets of bacterial lineages, one that hasbeen cultured at 37 C for 20,000 generations and another that will becultured at 37 C for 2,000 generations, after having adapted to otherthermal environments. The results of these studies will provide importantexperimental tests of predictions for the responses of biological systemsto global climate change and for general evolutionary theory concerningenvironmental adaptation.