An Automated DNA Sequencing and Genotyping Facility for Studies of Ecology, Systematics, Evolution, and Behavior

用于生态学、系统学、进化和行为研究的自动 DNA 测序和基因分型设备

• 批准号: 9970113
• 负责人: Richard Harrison
• 金额: $ 12.02万
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9970113&HistoricalAwards=false
• 关键词: Automated; DNA; Sequencing; Genotyping; Facility

ABSTRACT(DBI-9970113) Among population biologists, behavioral ecologists, conservation biologists, and systematists, there is increasing demand for molecular (DNA) markers that can provide information on mating systems, population structure, genealogy, and phylogeny. It is also becoming clear that extremely large data sets will often be required (1) to provide definitive answers about the forces that shape genetic change over time and space and (2) to achieve a clear understanding of relationships among individuals within species and among species or higher taxa. Automated DNA sequencing and genotyping have now improved to the point that they clearly can enhance the efficiency and productivity of research programs in ecology, evolutionary biology, conservation biology, systematics and behavior. Funds will be used to purchase an automated sequencer for a multi-user facility that will serve a large community of biologists within these disciplines. These biologists are part of four distinct units at Cornell University, but all contribute to what is arguably one of the premiere graduate programs in organismal biology in the country. The multi-user facility will not only provide access to cost-effective DNA sequencing and genotyping for faculty, students, and postdoctoral associates, but will markedly improve the training of students and catalyze interactions among scientists with diverse taxonomic and disciplinary interests.

摘要（DBI-9970113）在种群生物学家、行为生态学家、保护生物学家和系统分类学家中，对能够提供交配系统、种群结构、系谱和生殖系统信息的分子（DNA）标记的需求日益增加。 人们也越来越清楚，通常需要非常大的数据集来（1）提供关于随时间和空间形成遗传变化的力量的明确答案，（2）明确了解物种内个体之间以及物种或更高分类群之间的关系。 自动DNA测序和基因分型现在已经改进到这样一个程度，它们显然可以提高生态学、进化生物学、保护生物学、系统学和行为学研究项目的效率和生产力。 资金将用于购买多用户设施的自动测序仪，该设施将为这些学科内的大型生物学家社区提供服务。 这些生物学家是康奈尔大学四个不同单位的一部分，但都有助于什么可以说是在该国的有机体生物学首屈一指的研究生课程之一。 多用户设施不仅将为教师，学生和博士后提供具有成本效益的DNA测序和基因分型，而且将显着改善学生的培训，并促进具有不同分类学和学科兴趣的科学家之间的互动。