An Automated DNA Sequencing and Genotyping Facility for Studies of Ecology, Systematics, Evolution, and Behavior

摘要（DBI-9970113）在种群生物学家、行为生态学家、保护生物学家和系统学家中，对能够提供交配系统、种群结构、谱系和系统发育信息的分子（DNA）标记的需求日益增加。越来越清楚的是，通常需要极其庞大的数据集：(1)提供关于随时间和空间形成遗传变化的力量的明确答案；(2)对物种内个体之间、物种之间或更高分类群之间的关系有清晰的认识。自动化的DNA测序和基因分型技术现在已经进步到一定程度，它们明显可以提高生态学、进化生物学、保护生物学、系统学和行为学研究项目的效率和生产力。资金将用于为多用户设施购买自动测序仪，该设施将服务于这些学科内的大型生物学家社区。这些生物学家是康奈尔大学四个不同单位的一部分，但他们都为这个国家有机体生物学领域首屈一指的研究生项目做出了贡献。多用户设施不仅将为教师、学生和博士后提供具有成本效益的DNA测序和基因分型，而且将显著改善学生的培训，并促进具有不同分类和学科兴趣的科学家之间的互动。

ABSTRACT(DBI-9970113) Among population biologists, behavioral ecologists, conservation biologists, and systematists, there is increasing demand for molecular (DNA) markers that can provide information on mating systems, population structure, genealogy, and phylogeny. It is also becoming clear that extremely large data sets will often be required (1) to provide definitive answers about the forces that shape genetic change over time and space and (2) to achieve a clear understanding of relationships among individuals within species and among species or higher taxa. Automated DNA sequencing and genotyping have now improved to the point that they clearly can enhance the efficiency and productivity of research programs in ecology, evolutionary biology, conservation biology, systematics and behavior. Funds will be used to purchase an automated sequencer for a multi-user facility that will serve a large community of biologists within these disciplines. These biologists are part of four distinct units at Cornell University, but all contribute to what is arguably one of the premiere graduate programs in organismal biology in the country. The multi-user facility will not only provide access to cost-effective DNA sequencing and genotyping for faculty, students, and postdoctoral associates, but will markedly improve the training of students and catalyze interactions among scientists with diverse taxonomic and disciplinary interests.