Bioavailability of Carbon and Iron from Viral Lysis Products

病毒裂解产物中碳和铁的生物利用度

• 批准号: 9977040
• 负责人: Steven Wilhelm
• 金额: $ 24.31万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9977040&HistoricalAwards=false
• 关键词: Bioavailability; Carbon; Iron; Viral; Lysis

The investigator will examine the role of viruses in the biogeochemical cycling of iron (Fe) and carbon (C) in marine systems. Viruses are a major cause of prokaryotic mortality, but in spite of many suggestions that they are important in nutrient recycling, only minimal quantitative evaluations of this process exist. Preliminary data demonstrate that viruses can mediate rapid transfers of elements to bacteria and other plankton. As prokaryotic organisms represent the majority of the living biomass in the ocean, viral lysis of autotrophic and heterotrophic prokaryotes represents a little studied source of potentially limiting nutrients to marine organisms. Recent evidence also suggests that the bioavailability of Fe to marine organisms is regulated by the complexation of Fe by uncharacterized organic ligands in seawater. This project will directly measure virus-mediated release of Fe and C from host cells using radioisotope and ultrafiltration techniques, and examine subsequent Fe bioavailability. Field studies in the subtropical Atlantic Ocean and Strait of Georgia (British Columbia) will complement the lab studies by focussing on size-fractionated virus-host community dynamics and Fe/C transfer.

研究人员将研究病毒在海洋系统中铁（Fe）和碳（C）的地球化学循环中的作用。 病毒是原核生物死亡的主要原因，但尽管有许多建议，他们是重要的营养循环，只有最低限度的定量评估这一过程存在。 初步数据表明，病毒可以介导元素快速转移到细菌和其他浮游生物。 由于原核生物代表了海洋中的大部分生物量，因此自养和异养原核生物的病毒裂解代表了很少研究的潜在限制海洋生物营养物质的来源。 最近的证据还表明，铁的生物利用度的海洋生物是由铁的络合物的非特征性的有机配体在海水中。 该项目将使用放射性同位素和超滤技术直接测量病毒介导的Fe和C从宿主细胞中的释放，并检查随后的Fe生物利用度。 在亚热带大西洋和格鲁吉亚海峡（不列颠哥伦比亚省）的实地研究将补充实验室研究的重点是大小分级病毒宿主群落动态和Fe/C转移。