XYZ on a Chip: Electroactive Monolayers for Integrating Circuits with Mammalian Cells

9980850 ksich这个工程微系统：“XYZ”芯片项目的目标是开发一种基本的方法来集成混合设备中的细胞和材料组件的功能。在金上自组装的链烷硫酸酯单层，呈现肽配体，将被用作控制细胞与底物之间相互作用的模型底物。该项目将开发电活性单层膜，可以实时调节与附着细胞表面受体相互作用的固定配体的活性。该策略是基于电活性基团的设计，当电位施加到潜在的金时，这些基团会被氧化或还原。当这些电活性基团被结合到固定在单分子层上的配体结构中时，它们可以用来影响配体活性的变化。该程序将提供三种电活性单层膜，可用于控制附着细胞的行为。其中包括选择性释放固定配体的单分子膜，开启固定配体活性的单分子膜，以及可逆调节固定配体活性的单分子膜。一个相关的工作将是开发电活性配体，它可以报告细胞受体的结合，从而将生物信号转化为电输出。由于这类单层膜的设计目的是有效地将电信号转换为生物信号，因此它代表了成熟集成电路技术的附加能力。这里开发的策略可能对设计广泛的混合设备很重要，这些混合设备结合了细胞和材料组件，用于诊断、组织工程、留置传感器、神经计算和生物医学系统。

9980850MrksichThe goal of this Engineering Microsystems: "XYZ" on a Chip project will develop a fundamental approach for integrating the functions of cellular and materials components in hybrid devices. Self-assembled monolayers of alkanethiolates on gold that present peptide ligands will be used as model substrates that control the interactions between cells and the substrate. The program will develop electroactive monolayers that can modulate, in real time, the activity of immobilized ligands that interact with the surface receptors of attached cells. The strategy is based on the design of electroactive groups that undergo oxidation or reduction when electrical potentials are applied to the underlying gold. When these electroactive groups are incorporated into the structures of ligands immobilized to the monolayers, they can be used to effect changes in the activity of the ligands. This program will deliver three classes of electroactive monolayers that can be used to control the behaviors of attached cells. These include monolayers that selectively release immobilized ligands, monolayers that turn on the activity of immobilized ligands, and monolayers that reversibly modulate the activity of immobilized ligands. A related effort will develop electroactive ligands that can report on the binding of cellular receptors, and hence transduce biological signals into electrical outputs. Because this class of monolayers is designed to efficiently convert electrical signals into biological signals, it represents an add-on capability to the mature technology of integrated circuits. The strategies developed here may be important to designing a broad range of hybrid devices that combine cellular and materials components for use in diagnostics, tissue engineering, indwelling sensors, neural computation, and BioMEMS.