Fabrication of Integrated Polymeric Microfluidic Systems

本工作的主要目标是开发基于PDMS及其相关材料的功能性微流控装置。战略意图是开发尽可能简单的平台（即完整的系统，具有完成样品导入、制备、分离、检测和处理的组件）。我们将主要使用聚二甲基硅氧烷（PDMS）（和其他有机聚合物）制造集成微分析系统。这项工作将需要开发若干子系统和组件。这项工作的目标将是完成开发灵活平台技术的任务，并展示新的概念。更具体地说，这项工作将包括：i)建立PDMS的能力，凭借其灵活性和低表面自由能，使某些类型的功能成为可能；ii)扩大微流体系统设计人员可用的组件范围。重点将放在蛋白质组学而不是基因组学上。PI将探索这些想法或组件：层流和逆流作为分离的基础，用于细胞和蛋白质的图案和剂量，以及通道内制造。*新型功能部件：片上等效过滤器，透析室，磁性分选器，离子交换吸附器和膜，以及离心机（无移动部件）。*具有活动部件的组件：具有活动部件的新型组件，利用PDMS的柔韧性来消除铰链和使用硅时所需的其他复杂功能。*适用于芯片级实现的蛋白质分离和分析系统。

Whitesides0004030The principal objective in this work is to develop functional microfluidic devices based on PDMS and related materials. The strategic intent is to develop the simplest possible platform (meaning complete system, with components that accomplish sample introduction, preparation, separation, detection, disposal). We will fabricate integrated microanalytical systems using primarily poly(dimethylsiloxane) (PDMS) (and other organic polymers).The work will require development of a number of subsystems and components. The objectives of this work will be both to accomplish the task of developing a flexible platform technology, and to demonstrate new concepts. More specifically, the work will include: i) establishing the capability of PDMS to make certain types of functions uniquely possible by virtue of its flexibility and low surface free energies, and ii) expanding the range of components available to designers of microfluidic systems. The focus will be on proteomics rather than geonimcs.The PI will explore these ideas or components: * Laminar flow and counterflow as the basis for separations, for patterning and dosing cells and proteins, and for in-channel fabrication.* New types of functional components: on-chip equivalents of filters, dialysis chambers, magnetic sorters, ion exchange adsorbers and membranes, and centrifuges (with no moving parts).* Components with moving parts: new types of components with moving parts, taking advantage of the ability of PDMS to flex to eliminate hinges and other complex features that are required when using silicon.* Systems for separation and analysis of proteins that are amenable to chip-scale realization.