MEMS Biosensor Array for Biochemical and Clinical Diagnostics

用于生化和临床诊断的 MEMS 生物传感器阵列

• 批准号: 0097468
• 负责人: Srinivas Tadigadapa
• 金额: $ 40万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0097468&HistoricalAwards=false
• 关键词: MEMS; Biosensor; Array; Biochemical; Clinical

Calorimetry is a very versatile and effective investigative tool for analyzing biochemical reactions and is capable of providing continuous quantitative assays of biochemical reactions with exceptional operational stability. The ability to follow the progress of a biochemical reaction continuously as a function of time or reactant concentration has many research and clinical applications with far reaching consequences. However, calorimetric bioanalysis has been mostly hampered by the high cost, slow response times and cumbersome nature of the equipment. To address these limitations, a novel calorimetric biosensor is proposed by integrating a polymer based micro-reaction chamber on a MEMS thin film freestanding microthermopile sensor. The thermopile will be fabricated on a freestanding micromechanical structure such as a cantilever with the cold junctions placed on the bulk of the silicon chip (the rim) and the hot junctions on the freestanding structure. A polymer-based reaction chamber will be integrated right above the hot junctions of the thermopile. The large thermal mass and good thermal conductivity of the rim keeps the cold junctions at room temperature whereas the unconstrained cantilever tip will rise in temperature due to the heat of reaction. Coating the hot junctions with specific immobilized enzymes will provide selectivity by catalyzing only the reaction of the corresponding analyte. The sensor will also be used for the accurate measurement of the specific heat and thermal diffusivity of the reactants. Real-time monitoring of these thermal properties will provide with an independent tool for analyzing the progress of the biochemical reaction.

量热法是分析生化反应的一种非常通用和有效的研究工具，能够提供连续的生化反应定量分析，具有出色的操作稳定性。作为时间或反应物浓度的函数连续跟踪生化反应进程的能力具有许多研究和临床应用，具有深远的影响。然而，量热生物分析主要受到高成本、反应时间慢和设备笨重的阻碍。为了解决这些限制，提出了一种新的量热生物传感器，通过集成基于聚合物的微反应室的MEMS薄膜独立式微型热电堆传感器。热电堆将在独立的微机械结构上制造，例如悬臂梁，冷结放置在硅芯片的主体（边缘）上，热结放置在独立结构上。基于聚合物的反应室将集成在热电堆的热结上方。大的热质量和良好的导热性的边缘保持冷结在室温下，而不受约束的悬臂尖端将在温度上升，由于反应热。用特定的固定化酶涂覆热结将通过仅催化相应分析物的反应来提供选择性。该传感器还将用于准确测量反应物的比热和热扩散率。实时监测这些热特性将为分析生化反应的进展提供一个独立的工具。