I-Corps: Calorimetric Diagnostic Instrument for Acute Kidney Infection Monitoring

I-Corps：用于监测急性肾脏感染的量热诊断仪器

• 批准号: 1544180
• 负责人: Srinivas Tadigadapa
• 金额: $ 5万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1544180&HistoricalAwards=false
• 关键词: Corps; Calorimetric; Diagnostic; Instrument; Acute

Acute kidney injury (AKI) occurs in 5-7% of hospitalized patients and results in a mortality rate of about 50%. A particularly disturbing feature has been the inability since the mid-1960s to substantially lower mortality rates in AKI. The financial costs of AKI are estimated to be 8 billion dollars per year, or about $130,000 per life-year saved. It is unlikely that this high mortality and associated cost will be reduced until better tools for the early diagnosis of renal injury are available. Accordingly, a critical need exists to develop clinically efficient means for the detection of early acute renal failure. This NSF I-Corps project aims to explore and evaluate the commercialization potential of the "Calorimetric Diagnostic Instrument for Acute Kidney Infection" that has been engineered from an earlier NSF funded research proposal. Specifically, the proposed instrument involves a quartz resonator based thermal biosensor concept into a biomedical instrumentation system for continuous monitoring of kidney function based on the measurement of urinary creatinine excretion.Using the quartz based calorimetric device, the team has developed a biosensing system to quantify the concentration of creatinine in urine in a near continuous fashion. The biosensor consists of miniaturized pumps, quartz resonator sensor, electronics, and a replicable fluidic cartridge packed with immobilized enzyme. The idea of having the sensor and its electronics separated from the analyte of interest not only provides advantages in measurements but allows for significant cost savings. Rather, than having to throw away expensive sensors intertwined with the immobilized enzyme, the proposed operational scheme allows for the sensor system to be used indefinitely with only the fluidic tubing containing immobilized enzyme being easily swapped. Hence this design lends itself to a commercialization model akin to the glucose sensor. The team plans to summarize and incorporate the various suggestions from customer interviews during the I-Corps program to build a beta-prototype.

急性肾损伤（阿基）发生在5-7%的住院患者中，导致约50%的死亡率。一个特别令人不安的特征是自20世纪60年代中期以来无法大幅降低阿基的死亡率。阿基的财务成本估计为每年80亿美元，或每挽救一个生命年约13万美元。在早期诊断肾损伤的更好工具出现之前，这种高死亡率和相关费用不太可能降低。因此，迫切需要开发用于检测早期急性肾衰竭的临床有效手段。这个NSF I-Corps项目旨在探索和评估“急性肾脏感染热量诊断仪”的商业化潜力，该仪器是根据早期NSF资助的研究提案设计的。具体来说，该仪器将基于石英谐振器的热生物传感器概念引入生物医学仪器系统，用于基于尿肌酐排泄测量的连续监测肾功能。使用基于石英的量热装置，该团队开发了一种生物传感系统，以近乎连续的方式量化尿液中肌酐的浓度。该生物传感器由微型泵、石英谐振器传感器、电子器件和填充有固定化酶的可复制射流盒组成。使传感器及其电子器件与感兴趣的分析物分离的想法不仅在测量中提供优势，而且允许显著的成本节约。相反，不是必须丢弃与固定化酶缠绕的昂贵传感器，所提出的操作方案允许传感器系统无限期地使用，其中仅包含固定化酶的流体管被容易地交换。因此，这种设计适合于类似于葡萄糖传感器的商业化模型。该团队计划在I-Corps计划期间总结并整合客户访谈中的各种建议，以构建测试原型。