BAC Resources for Comparative Biology in Selected Angiosperms

BAC 选定被子植物比较生物学资源

• 批准号: 0208311
• 负责人: Andrew Paterson
• 金额: $ 40万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0208311&HistoricalAwards=false
• 关键词: BAC; Resources; Comparative; Biology; Selected

0208311PatersonBAC libraries for key branches of the flowering plant family tree will greatly accelerate progress in unraveling the 200-million year history of angiosperm evolution. Extensive prior investments in the Poales and Brassicales make them important nucleation points for the monocots and dicots, respectively, from which to reach out across plant diversity to further investigate evolution of genome size, gene repertoire and function, and plant morphology. We will make 5 libraries totaling 423,936 BACs from carefully-selected angiosperm genomes (see below) to empower new investigations of well-delineated questions in developmental, evolutionary, and comparative biology. Among these, two (Ananas, Opuntia) will be the first BAC libraries known in their taxonomic orders, and two will be the first for important genome types (A, F) within a species complex (Gossypium) that is especially facile for asking fundamental questions about morphological transformations, polyploid evolution, and genome organizationThese libraries will empower new investigations in monocots and dicots respectively, of general mechanisms associated with plant diversification, and the specific events that may account for major morphological-developmental transformations, by virtue of making possible important new comparisons to many taxa already well studied. These libraries will also offer opportunities to shed new light on the genomic consequences of polyploidy, and new insights into the developmental genetics of other scientifically important features that are inadequately studied at the molecular level. All of the taxa we will study are closely-related to economically-significant crops that exhibit features 'amplified' by human selection, and as such comprise botanical models in their own right for various aspects of plant growth and development.This work will empower many scientific synergies to be realized by links with prior investments by the Arabidopsis and Rice Genome Initiatives, NSF Plant Genome Program (in the Poales and Malvales), USDA-IFAFS (in the Brassicales), and USDA-NRI genome programs (Malvales). Community involvement in decisions relating to this project has been substantial. Our research is closely-tied to strong training and outreach programs with a successful history of engaging undergraduates and groups under-represented in the sciences.Binomial Common name Family 1C value (Mbp) # of BACs Genome coverage Ananas comosus pineapple Bromeliaceae 531 55,296 10xGossypium herbaceum A-genome cotton Malvaceae 1785 92,160 5xGossypium longicalyx F-genome cotton Malvaceae 1592 92,160 6xGossypioides kirkii Malvaceae 579 73,728 12xOpuntia cochellinifera prickly-pear cactus Cactaceae 869 110,592 10x TOTAL 423,936

[20208311]有花植物家族树关键分支的patsonbac库将极大地加速揭示2亿年被子植物进化历史的进程。在此之前，研究者们对扁豆科植物和芸苔科植物进行了大量的研究，使它们分别成为单子叶和双子叶植物的重要成核点，从而进一步研究植物多样性的基因组大小、基因库和功能以及植物形态的进化。我们将从精心挑选的被子植物基因组（见下文）中建立5个文库，总计423,936个bac，以支持对发育，进化和比较生物学中明确描述的问题进行新的研究。其中，两个（Ananas, Opuntia）将是已知的第一个分类顺序的BAC文库，两个将是物种复合体（棉属植物）中重要的基因组类型（A， F）的第一个文库，这些文库特别便于研究形态转化，多倍体进化和基因组组织的基本问题。这些文库将分别为单子房和双子房的新研究提供支持，研究与植物多样化相关的一般机制。通过与许多已经得到充分研究的分类群进行重要的新比较，可能解释主要形态发育转变的具体事件。这些文库还将为揭示多倍体的基因组结果提供新的机会，并为在分子水平上研究不足的其他科学重要特征的发育遗传学提供新的见解。我们将研究的所有分类群都与经济上重要的作物密切相关，这些作物表现出被人类选择“放大”的特征，因此它们本身就包含了植物生长和发育的各个方面的植物模型。这项工作将通过与拟南芥和水稻基因组计划、美国国家科学基金会植物基因组计划（Poales和Malvales）、USDA-IFAFS （brassicale）和USDA-NRI基因组计划（Malvales）的先前投资联系起来，实现许多科学协同效应。社区积极参与与这个项目有关的决策。我们的研究与强大的培训和推广项目密切相关，在吸引本科生和科学领域代表性不足的群体方面有着成功的历史。BACs的二项共同名称家族1C值（Mbp） #基因组覆盖率凤梨凤梨科531 55296 10xsssypium herbaceum a -基因组棉花Malvaceae 1785 92,160 5xsssypium longalyx f -基因组棉花Malvaceae 1592 92,160 6xssypioides kirkii Malvaceae 579 73,728 12xOpuntia cochellinifera刺梨仙人掌仙人掌仙人掌869 110,592 10x TOTAL 423,936