Microbial Genome Sequencing: Genome Sequencing of the Budding Bacterium Hyphomonas Neptunium

蒂莫西·R.格鲁吉亚大学的胡佛、印第安纳州大学的伊夫·V·布伦博士和基因组研究所的纳奥米·沃德博士获得了一笔赠款，用于对萌芽细菌海王星丝单胞菌的基因组进行测序。 该项目的目标是识别和分配潜在的功能，在H基因。镎 螺杆镎基因组由形成约2，000个基因的约270万个核苷酸碱基对组成。 将对随机基因组DNA片段进行测序，并将收集的数据组装成完整的基因组序列。 将使用算法进行基因鉴定，并通过将这些产物的序列与细菌蛋白质序列数据库中的序列进行比较来分配基因产物的潜在作用。 与数据库中的条目相匹配的基因将具有与分配给预测编码区的匹配相关联的相应作用和基因通用名称。许多细菌通过称为出芽的过程进行繁殖。 关于出芽或相关活动如染色体分配知之甚少。 了解这些过程对于开发控制有害细菌生长或利用具有理想活性的细菌的新策略至关重要。 这项研究将通过鉴定一个有代表性的出芽细菌中的所有基因来加强对出芽的研究。 研究人员可以利用这些数据来识别在出芽或相关过程中发挥作用的蛋白质。 有趣的是，一个新分化的柄细胞的出芽潜力仅限于大约7个出芽事件;镎可用作微生物衰老的模型。这样的实验可以进行调整，以适合本科生，研究生，或博士后研究人员。 因此，这项研究将为各种各样的学生提供重要的教育机会。 这是一个微生物基因组测序奖，通过国家科学基金会和农业部之间的合作计划资助。

Dr. Timothy R. Hoover of the University of Georgia, Dr. Yves V. Brun of Indiana University, and Dr. Naomi Ward of The Institute for Genomic Research have been awarded a grant to sequence the genome of the budding bacterium Hyphomonas neptunium. The goal of the project is to identify and assign potential functions to the genes in H. neptunium. The H. neptunium genome consists of approximately 2.7 million nucleotide base pairs forming about 2,000 genes. Random genomic DNA fragments will be sequenced, and the collected data will be assembled into the complete genomic sequence. Gene identification will be done using an algorithm and potential roles for the gene products will be assigned by comparing the sequences of these products with those in a database of bacterial protein sequences. Genes that match entries in the database will have the corresponding role and gene common name associated with the match assigned to the predicted coding region.Many bacteria reproduce through a process known as budding. Little is known about budding or associated activities such as chromosome partitioning. Understanding these processes is critical for developing new strategies for controlling the growth of unwanted bacteria or exploiting those with desirable activities. This research will enhance studies on budding by identifying all of the genes within a representative budding bacterium. Researchers can use these data to identify proteins that have roles in budding or associated processes. Interestingly, the budding potential of a newly differentiated stalk cell is limited to about seven budding events; thus H. neptunium can be used as a model for microbial senescence. Such experiments can be adapted to be appropriate for undergraduates, graduate students, or post-doctoral researchers. Thus, this research will provide significant educational opportunities for a wide variety of students. This is a Microbial Genome Sequencing Award funded through a collaborative program between the National Science Foundation and the Department of Agriculture.