Microbial Genome Sequencing: Genome Sequencing of the Budding Bacterium Hyphomonas Neptunium

微生物基因组测序：出芽细菌 Hyphomonas Neptunium 的基因组测序

• 批准号: 0237224
• 负责人: Timothy Hoover
• 金额: $ 32.5万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0237224&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Budding; Bacterium

Dr. Timothy R. Hoover of the University of Georgia, Dr. Yves V. Brun of Indiana University, and Dr. Naomi Ward of The Institute for Genomic Research have been awarded a grant to sequence the genome of the budding bacterium Hyphomonas neptunium. The goal of the project is to identify and assign potential functions to the genes in H. neptunium. The H. neptunium genome consists of approximately 2.7 million nucleotide base pairs forming about 2,000 genes. Random genomic DNA fragments will be sequenced, and the collected data will be assembled into the complete genomic sequence. Gene identification will be done using an algorithm and potential roles for the gene products will be assigned by comparing the sequences of these products with those in a database of bacterial protein sequences. Genes that match entries in the database will have the corresponding role and gene common name associated with the match assigned to the predicted coding region.Many bacteria reproduce through a process known as budding. Little is known about budding or associated activities such as chromosome partitioning. Understanding these processes is critical for developing new strategies for controlling the growth of unwanted bacteria or exploiting those with desirable activities. This research will enhance studies on budding by identifying all of the genes within a representative budding bacterium. Researchers can use these data to identify proteins that have roles in budding or associated processes. Interestingly, the budding potential of a newly differentiated stalk cell is limited to about seven budding events; thus H. neptunium can be used as a model for microbial senescence. Such experiments can be adapted to be appropriate for undergraduates, graduate students, or post-doctoral researchers. Thus, this research will provide significant educational opportunities for a wide variety of students. This is a Microbial Genome Sequencing Award funded through a collaborative program between the National Science Foundation and the Department of Agriculture.

佐治亚大学的Timothy R. Hoover博士、印第安纳大学的Yves V. Brun博士和基因组研究所的Naomi Ward博士获得了一项资助，用于对萌发中的细菌neptium菌丝单胞菌的基因组进行测序。该项目的目标是鉴定和分配H.镎基因的潜在功能。H.镎基因组由大约270万个核苷酸碱基对组成，形成大约2000个基因。随机的基因组DNA片段将被测序，收集到的数据将被组装成完整的基因组序列。基因鉴定将使用算法完成，基因产物的潜在作用将通过将这些产物的序列与细菌蛋白质序列数据库中的序列进行比较来分配。与数据库条目相匹配的基因将具有相应的角色和与匹配相关联的基因公共名称分配给预测编码区域。许多细菌通过一种称为出芽的过程进行繁殖。对出芽或相关活动如染色体分裂知之甚少。了解这些过程对于开发新的策略来控制不需要的细菌的生长或开发具有理想活性的细菌至关重要。本研究将通过鉴定一个有代表性的出芽细菌的所有基因来加强对出芽的研究。研究人员可以利用这些数据来识别在出芽或相关过程中起作用的蛋白质。有趣的是，一个新分化的柄细胞的出芽潜能被限制在大约7个出芽事件；因此，H.镎可以作为微生物衰老的模型。这样的实验可以适应适合本科生，研究生，或博士后研究人员。因此，这项研究将为各种各样的学生提供重要的教育机会。这是一个微生物基因组测序奖，由国家科学基金会和农业部合作项目资助。