Microbial Genome Sequencing: Complete Genome Sequencing of the Extremely Halophilic Archaea Haloferax Volcanii

微生物基因组测序：极嗜盐古菌 Haloferax Volcanii 的完整基因组测序

• 批准号: 0243492
• 负责人: Jonathan Eisen
• 金额: $ 24.72万
• 依托单位: Institute for Genomic Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0243492&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Complete; Extremely

The primary goal of this project is to sequence to completion the 4.2 megabase genome of the extremely halophilic Archaea, Haloferax volcanii and to aid scientists and educators in making use of this data. This project can be divided into four distinct aims: (1) to carry out whole-genome shotgun sequencing, assembly, and closure of the genome of H. volcanii; (2) to facilitate community annotation using the TIGR MANATEE software and analyze the genome sequence (3) to establish a user-friendly access to the H. volcanii genome sequence and to the genome annotation and analysis, by the general biological and biomedical research community; (4) to aid science educators in the development of tools for using this species as a model for genome-enabled microbiology education. Extreme halophilic Archaea are uniquely able to survive in high salt environments because they balance the internal salt concentration with that of the external environment and because they have adapted their intercellular macromolecular components (proteins, nucleic acids, etc.) to function a high salt milieu. H. volcanii is an excellent model system for the study of the general biology of Archaea. It is easily cultivated at moderate temperatures and is amenable to genetic and molecular manipulation. This organism, in addition to being salt dependent, is desiccation and radiation resistant and is being used as a model to study novel metabolic pathways, adaptation to changing salinity and for heterologous expression of gas vacuole and bacterio-opsin encoding genes. Only one complete genome is currently available from this group of halophilic Archaea. Having multiple genomes from this group will allow comparative genomic analysis which has been found to provide remarkable insight into the evolution, mutation, and biology of microbial species. Because of its ease of growth, non pathogenic character and interesting biological properties, we believe H. volcanii can become a model for undergraduate science education courses.

该项目的主要目标是完成极端嗜盐菌Haloferax volcanii的420万个基因组的测序，并帮助科学家和教育工作者利用这些数据。该项目主要有四个目标：（1）进行全基因组鸟枪法测序，组装和封闭H. volcanii;（2）利用TIGR MANATEE软件进行社区注释和基因组序列分析;（3）建立一个用户友好的H. volcanii基因组序列以及基因组注释和分析;（4）帮助科学教育工作者开发工具，将该物种用作基因组微生物学教育的模式。极端嗜盐菌是唯一能够在高盐环境中生存的，因为它们平衡了内部盐浓度与外部环境，因为它们已经适应了细胞间的大分子成分（蛋白质，核酸等）。以适应高盐环境。H. volcanii是一个很好的模式系统，用于研究海洋生物学。它很容易在中等温度下培养，并适合遗传和分子操作。这种生物体，除了盐依赖性，是干燥和辐射抗性，并被用作模型，研究新的代谢途径，适应不断变化的盐度和异源表达的气泡和细菌视蛋白编码基因。目前只有一个完整的基因组是从这个组的嗜盐菌。具有来自该组的多个基因组将允许进行比较基因组分析，这已经被发现为微生物物种的进化、突变和生物学提供了显着的见解。由于其易于生长、无致病性和有趣的生物学特性，我们认为H. volcanii可以成为本科科学教育课程的典范。