UHPLC-coupled Tandem Quadrupole Mass Spectrometer for Multiple Reaction Monitoring measurements

将在汉堡大学生物系建立一个用于多反应监测测量的UHPLC耦合串联四极杆质谱仪，用于对不同生化基质中的定义化合物进行灵敏和高选择性的定量。为了在样品进入质谱仪（MS）之前降低样品的复杂性，并以具有高化学变化的广泛分析物为目标，将在MS之前使用液相色谱耦合。一个主要的应用将是确定植物-微生物以及藻类-微生物相互作用在有机物周转和碳通量中的作用。需要研究的主要基质是根际，植物根系和微生物之间的复杂相互作用有助于土壤有机质的转化和降解。将在沿海生态系统、温带森林和湿地中研究热带球。将研究海洋环境中浮游植物分泌物的作用和浮游植物-微生物的相互作用。此外，还将研究植物凋落物中的碳水化合物周转和生物膜中的关键代谢物。除了专注于碳通量的项目外，该仪器还将用于各种其他应用，例如分析高度专业化的食草昆虫中的cardenoprotein含量，定量C.线虫，人类类固醇激素滴度的定量，以及鱼类胚胎中靶向代谢物的定量。该仪器将成为汉堡大学和汉堡-艾本德大学医学中心技术平台质谱仪的一部分。将编写仪器专用标准操作规程并提供给所有用户。对仪器的访问将通过MS技术平台的用户规则进行管理，该规则优先考虑内部用户，而不是外部学术用户。核心设施管理软件将帮助安排测量时间。测量期间数据将本地保存，并直接镜像到仪器操作计算机的第二个硬盘驱动器上。对于永久数据存储，服务器容量将由汉堡大学通过其区域计算中心提供。

An UHPLC-coupled Tandem Quadrupole Mass Spectrometer for Multiple Reaction Monitoring measurements will be established in the Department of Biology at Universität Hamburg for sensitive and highly selective quantification of defined compounds out of different biochemical matrices. To reduce the complexity of the sample prior to its entrance into the Mass Spectrometer (MS) and to target a broad range of analytes with a high chemical variety, Liquid Chromatography coupling prior to MS will be used. One major application will be the determination of the role of plant-microbe- as well as algae-microbe-interactions in organic matter turnover and carbon fluxes. A major matrix to be studied is the rhizosphere, where complex interactions between plant roots and microbes contribute to soil organic matter transformation and degradation. Rhizospheres will be studied in coastal ecosystems, temperate forests, and wetlands. The role of phytoplankton exudates and phytoplankton-microbe interactions will be studied in marine environments. In addition, carbohydrate turn-over in plant litter and key-metabolites in biofilms will be studied. Beside projects focusing on carbon fluxes, the instrument will also be used in variety of other applications as e.g. the analysis of cardenolide contents in highly specialized herbivorous insects, the quantification of TCA cycle intermediates in C. elegans, the quantification of steroid hormone titers in humans, and the quantification of targeted metabolites in fish embryos. The instrument will be part of the Technology Platform Mass Spectrometry of Universität Hamburg and the University Medical Center Hamburg-Eppendorf. Instrument specific Standard Operating Procedures will be written and provided to all users. Access to the instrument will be regulated via user rules of the MS technology platform, which prioritize internal users over external academic users over other external users. The core facility management software will help scheduling measurement times. Data will be locally saved during the measurement and mirrored directly on a second hard drive in the operating computer of the instrument. For permanent data storage, server capacities will be provided by Universität Hamburg through its Regional Computing Center.