ShEEP Request for Nikon N-Storm Super-resolution microscope

ShEEP 请求尼康 N-Storm 超分辨率显微镜

• 批准号: 9795345
• 负责人: Ann C. McKee
• 金额: --
• 依托单位: VA BOSTON HEALTH CARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795345
• 关键词: 3-Dimensional; Aging; Alzheimer' s Disease; Area; Biological Markers; Biological Models; Boston; Cell model; Cells; Dementia; Dimensions; Disease; Ensure; Epilepsy; Fluorescent in Situ Hybridization; Funding; Genetic Transcription; Healthcare; Healthcare Systems; Image; Imaging Techniques; In Situ Hybridization; Individual; Lateral; Lighting; Messenger RNA; Methods; Microscope; Microscopy; Molecular; Nanoscopy; Nerve Degeneration; Neurodegenerative Disorders; Optics; Pathologic; Pathology; Phase; Positioning Attribute; Process; Production; Proteins; Research; Research Personnel; Research Project Grants; Research Support; Resolution; Sheep; Specimen; Speed; Structure; System; Techniques; Time; Tissue Sample; Tissues; Transcript; Traumatic Brain Injury; age related; chronic traumatic encephalopathy; experimental study; fluorescence microscope; improved; in vivo; instrumentation; microscopic imaging; molecular scale; nanometer; nanoscale; neuronal circuitry; pre-clinical; programs; reconstruction; two-dimensional

The VA Boston Healthcare System (VABHS) is requesting the purchase of a state-of-the-art Nikon N-Storm super-resolution microscope. This microscope can resolve objects the size of individual molecules. The system can be used to create images of pathological proteins in neurodegenerative diseases like chronic traumatic encephalopathy (CTE), Alzheimer’s disease (AD), and other aging-related neurodegenerative disorders. These images can be used to better understand how pathological molecules spread from cell to cell. It can also be used to study the cellular dynamics of epilepsy and traumatic brain injury in cellular model systems. In addition, new advances have made it possible to use the incredible resolution of the N-Storm microscope to quantify transcriptional expression of individual cells, thereby understand how neurodegenerative diseases alter transcriptional expression. Understanding what molecules are involved in neurodegeneration will provide biomarkers to identify diseases in the preclinical phases and ultimately develop better therapies for neurodegenerative disease. The capabilities of this system are not available within VABHS and are critical to ongoing research projects

VA波士顿医疗保健系统（VABHS）正在请求购买最先进的尼康相机 N-Storm超分辨率显微镜。这台显微镜能分辨个体大小的物体 分子。该系统可用于创建病理蛋白质的图像， 神经退行性疾病如慢性创伤性脑病（CTE）、阿尔茨海默病 (AD)和其他与衰老相关的神经退行性疾病。这些图像可以用来更好地 了解病理分子如何在细胞间传播。它也可以用来研究 癫痫和创伤性脑损伤的细胞动力学的细胞模型系统。此外，本发明还提供了一种方法， 新的进展使得使用N-风暴显微镜的难以置信的分辨率成为可能 量化单个细胞的转录表达，从而了解 神经变性疾病改变转录表达。了解分子是什么 参与神经退行性变的研究将提供生物标志物，以在临床前 阶段，并最终开发出更好的治疗神经退行性疾病的方法。的能力 VABHS中没有这种系统，这对正在进行的研究项目至关重要