Transcriptomic plasticity of maize hybrids and their parental inbred lines at the interface of genotype and development

玉米杂交种及其亲本自交系在基因型和发育界面的转录组可塑性

• 批准号: 166916654
• 负责人: Professor Dr. Frank Hochholdinger
• 金额: --
• 依托单位: Lehrstuhl für Crop Functional Genomics
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/166916654?language=en
• 关键词: Transcriptomic; plasticity; maize; hybrids; their

Commercial maize production is almost exclusively hybrid-based, because maize hybrids perform significantly better than their homozygous parents. Nevertheless, the molecular basis of this phenomenon, known as heterosis, remains largely enigmatic.In the first funding period we discovered a specific form of complementation of gene expression that we designated single parent expression (SPE). SPE-genes are expressed in only one of the two parental inbred lines but in both reciprocal hybrids. In maize root tissues we discovered >2.300 SPE-genes. As a consequence of SPE, in all root tissues under analysis, the total number of active genes in hybrids exceeded the number of genes expressed in their parental inbred lines by >400. Moreover, non-syntenic genes which emerged only after the last whole genome duplication of maize were significantly overrepresented among SPE genes.In the second funding period, we plan to extend these analyses by surveying the influence of genotype and development on the transcriptomic plasticity of maize hybrids by RNA-Sequencing experiments. The genotype-dependent control of transcriptomic plasticity in maize primary roots will be studied in six crosses of the recurrent female parent B73 with the inbred lines A554, H84, H99, OH43, W64A and Mo17. Developmental control of transcriptomic complexity will be monitored in these genotypes at three developmental stages defined by the length of primary roots. A major focus of the planned experiments will be to further explore the prevalence and role of SPE patterns during the manifestation of heterosis in maize primary roots and the identification of novel genotype specific SPE patterns. Moreover, the evolutionary origin of these genes, teir prevalence among classical maize genes identified by their visible mutant phenotype, their regulation and their function will be surveyed. Furthermore, differential, non-additive and allele-specific gene expression patterns will be determined to identify conserved and unique patterns between these genotype combinations during development. Finally, these expression patterns will be correlated with phenotypic and histological data to gain initial insights into the role of gene expression patterns in the developmental manifestation of heterosis in maize roots. These correlations with a relative small number of genotypes are expected to be relatively weak but might provide first cues for future research directions using larger panels of genotypes.In summary, the analysis of the genotypic and developmental control of the transcriptomic plasticity of maize hybrids and their parental inbred lines will provide insights into the complex molecular processes leading to heterosis manifestation in maize roots.

商业玉米生产几乎完全基于杂交，因为玉米杂交种的表现明显优于其纯合亲本。然而，这种现象的分子基础，即所谓的杂种优势，在很大程度上仍然是个谜。在第一个资助期，我们发现了一种特殊的基因表达互补形式，我们将其命名为单亲表达（SPE）。SPE基因仅在两个亲本近交系中的一个中表达，但在两个互交杂种中表达。在玉米根组织中，我们发现了>2.300个SPE基因。作为SPE的结果，在分析的所有根组织中，杂种中活性基因的总数超过其亲本近交系中表达的基因的数量>400。此外，非共线基因出现后，最后一个全基因组复制的玉米显着overrepresented SPE generations.In第二个资助期，我们计划扩大这些分析，调查基因型和发展的影响，转录可塑性的玉米杂交种的RNA测序实验。以轮回母本B73与自交系A554、H84、H99、OH43、W64A和Mo17的6个杂交组合为材料，研究了玉米初生根转录组可塑性的基因型依赖控制。将在这些基因型中监测转录组复杂性的发育控制，在三个发育阶段由初生根的长度定义。计划的实验的一个主要重点将是进一步探索SPE模式的流行和作用在玉米初生根杂种优势的表现和新的基因型特异性SPE模式的识别。此外，这些基因的进化起源，在经典玉米基因中的流行程度，其可见的突变表型，其调控和功能将进行调查。此外，将确定差异、非加性和等位基因特异性基因表达模式，以鉴定发育期间这些基因型组合之间的保守和独特模式。最后，这些表达模式将与表型和组织学数据，以获得初步的见解基因表达模式的作用，在玉米根的杂种优势的发展表现。这些相关性与一个相对较小的基因型，预计将是相对较弱的，但可能提供第一线索，为未来的研究方向，使用较大的面板genotype.In总之，基因型和发育控制的转录组可塑性的玉米杂交种及其亲本自交系的分析将提供深入了解复杂的分子过程，导致杂种优势表现在玉米根。

项目成果

Single-Parent Expression Is a General Mechanism Driving Extensive Complementation of Non-syntenic Genes in Maize Hybrids

• DOI: 10.1016/j.cub.2017.12.027
• 发表时间: 2018-02-05
• 期刊: CURRENT BIOLOGY
• 影响因子: 9.2
• 作者: Baldauf, Jutta A.;Marcon, Caroline;Hochholdinger, Frank
• 通讯作者: Hochholdinger, Frank

Transcriptomic Dissection of Maize Root System Development

• DOI: 10.1007/978-3-319-97427-9_15
• 发表时间: 2018
• 作者: Peng Yu;Caroline Marcon;Jutta A. Baldauf;Felix P. Frey;Marcel Baer;F. Hochholdinger