Methodology for Rapid NMR Data Acquisition in Structural Genomics

该项目侧重于通过扩展适用于13C/15N稳定同位素标记的蛋白质和核酸的GFT核磁共振实验库，以及开发包含单扫描采集方案的GFT核磁共振实验，实现快速收集多维核磁共振数据的新方法。为了进一步减少GFT核磁共振的采样需求，将采用最大熵重构或三向分解对GFT核磁共振数据进行优化处理。用户友好的使用新的核磁共振实验将确保实现软件的有效分析GFT核磁共振光谱。核磁共振参数集包的创建将使研究结果的有效传播成为可能。这些活动产生的更广泛的影响是提高了基于核磁共振的结构生物学和基因组学的效率，并增加了对昂贵的高端核磁共振硬件和基因组测序项目的投资回报。这个研究项目将为学生提供机会。

This project focuses on the implementation of novel methodology for rapid collection of multidimensional NMR data by extending the repertoire of GFT NMR experiments suitable for 13C/15N stable isotope labeled proteins and nucleic acids and developing GFT NMR experiments that incorporate the single scan acquisition scheme. To further reduce the sampling demand of GFT NMR, processing of GFT NMR data will be optimized by using either Maximum Entropy Reconstruction or Three-way Decomposition. User-friendly employment of the new NMR experiments will be ensured by implementing software for efficient analysis of GFT NMR spectra. The creation of a package of NMR parameter sets will enable efficient dissemination of research results. The broader impacts resulting from these activities are increased efficiency with which NMR-based structural biology and genomics can be pursued and an increase in the return of investment into costly high-end NMR hardware and genome sequencing projects. The research program will offer opportunities to students.