Sensors: On-sensor Self-Calibration of Oxygen Microsensor; A Model System of Structural and Functional Integration of Biomicrosystem

标题：传感器：氧微传感器的传感器自校准；生物微系统结构与功能整合的模型系统。摘要本工作的目的是利用集成电化学驱动微系统实现溶解氧微传感器的片上内置智能控制（两点自校准/自诊断）。智能自主生化微传感器的发展尚处于起步阶段。为了克服由基线漂移和灵敏度下降引起的信号不稳定性，需要开发一种新的方案。我们建议使用电化学驱动机制（水电解）来实现这一新功能。水电解微系统（微室，微电极和微通道）将被设计为提供两个关键功能。首先，电解液与电化学产生的溶解气体相饱和，作为集成微传感器的校准剂（含氧饱和和贫氧电解质的两点程序）。其次，电化学生成的气泡为样品溶液的取样/分配提供了双向微流体操作的驱动力。采用微加工技术制备了一种包括安培溶解氧微传感器和水电解微致动器的微系统。它们的性能将根据驱动信号（持续时间和振幅）和微流控元件的几何形状来表征。预计自校准/自诊断能力将大大提高生物化学传感器在连续使用过程中的准确性和可靠性，并使生物和医学监测中的现场决策过程可行。电化学驱动是基于mems的微传感器技术中的一个新兴领域。该方法首次实现了生物化学传感器/执行器的功能集成（内置智能）与结构集成（小型化）相结合。电解驱动可用于提高其他利用氧化酶酶反应的酶微传感器的性能，实现智能光学生物传感系统。同样的方法可以实现微流控驱动，而不需要复杂的mems结构。这种微传感器和微致动器的系统集成预计将推动当前的生物微系统技术向创新仪器发展，用于自主和最少参与的生化监测。拟议活动产生的更广泛影响PI在密苏里大学罗拉分校（UMR）的电气与计算机工程系和生物科学系获得联合任命。PI的主要职责是通过发起全校范围内的跨学科研究活动和教育两个系的学生来弥合学科之间的差距。UMR是密苏里州路易斯·斯托克斯少数民族参与联盟（LS-MoAMP）项目的成员，并运行WIES（女性参与工程和科学项目）。拟议的研究将是宝贵的资源，通过来自不同学术部门和代表性不足群体的学生的参与，加快这些活动。

TITLE: Sensors: On-sensor self-calibration of oxygen microsensor; a model system of structuraland functional integration of biomicrosystem.ABSTRACTThe objective of this work is the implementation of on-chip, built-in intelligent control (2-pointself-calibration/self-diagnosis) of dissolved oxygen microsensor by using an integratedelectrochemical actuation microsystem. The development of intelligent and autonomousbiochemical microsensor is in infant stage. A novel protocol needs to be developed to overcomethe signal instability caused by drift of baseline and degradation of sensitivity. We propose touse an electrochemical actuation mechanism (water electrolysis) to accomplish this novelfunctionality. A water electrolysis microsystem (microchambers, microelectrodes, andmicrochannels) will be designed to provide two critical functions. First, the electrolytes saturatedwith the electrochemically generated dissolved gases serve as the calibrants for an integratedmicrosensor (two-point procedure with oxygen-saturated and oxygen-depleted electrolytes).Secondly, the electrochemically generated bubbles provide a driving force of the bidirectionalmicrofluidic manipulation for the sampling/dispensing of sample solution. A microsystemincluding an amperometric dissolved oxygen microsensor and a water electrolysis microactuatoris prepared by microfabrication technology. Their performances will be characterized in terms ofthe actuation signal (duration and amplitude) and the geometries of microfluidic components.The self-calibration/self-diagnosis capability is expected to significantly improve the accuracyand reliability of the biochemical sensors during continuous use and to make the in situ decision-making process viable in biological and medical monitoring.INTELLECTUAL MERITS OF PROPOSED ACTIVITYThe electrochemical actuation is an emerging field in the MEMS-based microsensortechnologies. The proposed method is the first approach to accomplish the functional integration(built-in intelligence) combined with the structural integration of biochemical sensor/actuator(miniaturization). The electrolysis actuation is applicable to improve performances of otherenzymatic microsensors that utilize the oxidase enzyme reaction and to accomplish intelligentoptical biosensing system. The same method can achieve the microfluidic actuation without thecomplicated MEMS-based structures. This system integration of both microsensors andmicroactuators is anticipated to advance current biomicrosystem technology towards innovativeinstrumentation for autonomous and minimally-attended biochemical monitoring.BROADER IMPACTS RESULTING FROM THE PROPOSED ACTIVITYThe PI has a joint appointment in the Departments of Electrical & Computer Engineering andBiological Sciences at the University of Missouri-Rolla (UMR). The primary responsibility ofthe PI is to bridge the gap between the disciplines by initiating the campus-wide interdisciplinaryresearch activities and by educating the students from both departments. The UMR is a memberof LS-MoAMP (The Louis Stokes Missouri Alliance for Minority Participation) program andruns WIES (Women in Engineering and Science Program). The proposed research will be avaluable resource to expedite these activities through the involvement of students from variousacademic departments and from underrepresented groups.