Molecular basis of Arabidopsis susceptibility to Pseudomonas syringae infection

拟南芥对丁香假单胞菌感染易感性的分子基础

基本信息

  • 批准号:
    0444915
  • 负责人:
  • 金额:
    $ 56万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2005
  • 资助国家:
    美国
  • 起止时间:
    2005-01-15 至 2009-12-31
  • 项目状态:
    已结题

项目摘要

In the co-evolution between plants and microbial pathogens, most plants have developed sophisticated immune responses to ward off the majority of (but not all) potential pathogens. When a plant fails to defend and the pathogen carries appropriate positive virulence factors, disease occurs. In the past two decades, significant efforts have been invested in studying how plants defend against pathogens at the molecular level. In contrast, little is known about how a plant fails to defend and ultimately succumbs to infection by virulent pathogens. The long-term goal of this project is to contribute to the understanding of the molecular basis of Arabidopsis susceptibility to bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000. Research on susceptible plant-pathogen interactions in the past two decades (mainly from the pathogen side) has revealed a wealth of information about intricate mechanisms used by pathogens to attack host plants. It is now clear that the majority of bacterial pathogens use a specialized protein secretion system-the type III secretion system-to actively 'inject' toxic effector proteins into the host cell. Mutations affecting this protein secretion system eliminate the ability of Pst DC3000 to infect Arabidopsis, suggesting a crucial role of type III effector proteins in modulating Arabidopsis susceptibility. Recent research provides exciting evidence that some type III effector proteins function as inhibitors of various forms of host immunity. For example, effector HopPtoM has recently been shown to inhibit salicylic acid-mediated host basal immunity and, directly or indirectly, to promote host cell death in Arabidopsis. However, the precise mechanisms by which the type III effector proteins modulate host immunity and other host cellular processes are not understood and remain a fundamental question in biology. Molecular genetic, cell biological, genomic, and transgenic experiments described in this grant will identify and characterize Arabidopsis cellular targets of effector HopPtoM. Understanding HopPtoM-mediated suppression of plant immunity and promotion of host cell death likely has broad implications for understanding bacterial pathogenesis in diverse plants. HopPtoM is functionally redundant to another effector, AvrE. Effectors of the HopPtoM and AvrE families are widespread in P. syringae strains and/or other plant-pathogenic bacteria. Most importantly, mutations in the HopPtoM/AvrE families of type III effectors often result in drastic loss of pathogen virulence. Elucidation of the mechanisms by which HopPtoM functions to inactivate the host defenses and to promote host cell death will therefore represent a significant step forward in our understanding of the molecular basis of plant susceptibility to bacterial pathogens. The proposed research also has a broader impact on society. This project will provide training of undergraduate, graduate, and postdoctoral scientists; it will broaden the participation in research of underrepresented groups; it will enhance the PI's teaching activities; and it will enable timely dissemination of knowledge through publications in scientific journals and presentations at regional, national and international conferences and to legislative officers. The basic knowledge gained from this project will provide a foundation for invention of novel strategies for controlling plant diseases, thus improving food quality and yield, reducing use of hazardous chemicals, and increasing national biosecurity and food safety.
在植物和微生物病原体之间的共同进化中,大多数植物已经发展出复杂的免疫反应来抵御大多数(但不是全部)潜在病原体。 当植物不能防御而病原体携带适当的正毒力因子时,疾病发生。 在过去的二十年里,人们投入了大量的精力来研究植物如何在分子水平上防御病原体。 相比之下,人们对植物如何无法防御并最终屈服于有毒病原体的感染知之甚少。 该项目的长期目标是有助于了解拟南芥对细菌病原体假单胞菌的易感性的分子基础。番茄(Pst)DC 3000. 在过去的二十年中,对易感植物-病原体相互作用的研究(主要从病原体方面)揭示了病原体攻击宿主植物的复杂机制的丰富信息。 现在很清楚,大多数细菌病原体使用专门的蛋白质分泌系统-III型分泌系统-将毒性效应蛋白主动“注入”宿主细胞。 影响这种蛋白质分泌系统的突变消除了Pst DC 3000感染拟南芥的能力,这表明III型效应蛋白在调节拟南芥易感性中起着至关重要的作用。 最近的研究提供了令人兴奋的证据,一些III型效应蛋白作为抑制剂的各种形式的宿主免疫功能。 例如,最近已经显示效应子HopPtoM抑制水杨酸介导的宿主基础免疫,并且直接或间接地促进拟南芥中的宿主细胞死亡。 然而,III型效应蛋白调节宿主免疫和其他宿主细胞过程的确切机制尚不清楚,仍然是生物学中的一个基本问题。 分子遗传学,细胞生物学,基因组学和转基因实验中描述的授权将确定和表征拟南芥细胞的目标效应HopPtoM。 理解HopPtoM介导的植物免疫抑制和促进宿主细胞死亡可能对理解不同植物中的细菌致病机制具有广泛的意义。 HopPtoM在功能上对另一个效应器AvrE是冗余的。 HopPtoM和AvrE家族的效应子广泛存在于甜菜夜蛾菌株和/或其他植物病原性细菌中。 最重要的是,III型效应子的HopPtoM/AvrE家族中的突变通常导致病原体毒力的急剧丧失。 因此,阐明HopPtoM发挥作用以破坏宿主防御并促进宿主细胞死亡的机制将代表我们在理解植物对细菌病原体易感性的分子基础方面向前迈出的重要一步。 这项研究也对社会产生了更广泛的影响。 该项目将为本科生、研究生和博士后科学家提供培训;它将扩大代表性不足群体参与研究的范围;它将加强公共研究所的教学活动;它将通过在科学期刊上发表文章和在区域、国家和国际会议上以及向立法官员介绍情况,及时传播知识。 从该项目中获得的基本知识将为发明控制植物病害的新战略奠定基础,从而提高粮食质量和产量,减少有害化学品的使用,并提高国家生物安全和食品安全。

项目成果

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Sheng He其他文献

Study of the mechanism by which magnetization reduces dust suppressant usage
磁化减少抑尘剂使用的机理研究
  • DOI:
    10.1016/j.colsurfa.2018.08.034
  • 发表时间:
    2018-12
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Hetang Wang;Sheng He;Guangran Xie;Xinyi Chen;Botao Qin
  • 通讯作者:
    Botao Qin
Loss and enhancement of layer-selective signals in geniculostriate and corticotectal pathways of adult human amblyopia
成人弱视膝纹和皮质顶盖通路中层选择性信号的丢失和增强
  • DOI:
    10.1016/j.celrep.2021.110117
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    8.8
  • 作者:
    Wen wen;Yue Wang;Jiawei Zhou;Sheng He;Xinghuai Sun;Rong Liu;Chen Zhao;Peng Zhang
  • 通讯作者:
    Peng Zhang
Investigations on THM effects in buffer , EDZ and argillaceous host rock Final Report
缓冲区、EDZ和泥质主岩中THM效应的研究最终报告
  • DOI:
  • 发表时间:
    2014
  • 期刊:
  • 影响因子:
    0
  • 作者:
    N. H. Lisssn;Sheng He
  • 通讯作者:
    Sheng He
A short-lived but significant Mongol–Okhotsk collisional orogeny in latest Jurassic–earliest Cretaceous
晚侏罗世—早白垩世的一次短暂但意义重大的蒙古—鄂霍茨克碰撞造山运动
  • DOI:
    10.1016/j.gr.2014.09.010
  • 发表时间:
    2015-10
  • 期刊:
  • 影响因子:
    6.1
  • 作者:
    Yong-Tai Yang;Zhi-Xin Guo;Chuan-Chun Song;Xiang-Bo Li;Sheng He
  • 通讯作者:
    Sheng He
Top-down modulation and cortical-AMG/HPC interaction in familiar face processing
熟悉面孔处理中的自上而下调制和皮质-AMG/HPC 交互
  • DOI:
    10.1101/2022.04.14.488206
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Xiaoxu Fan;Q. Guo;Xinxin Zhang;Ling;Sheng He;X. Weng
  • 通讯作者:
    X. Weng

Sheng He的其他文献

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{{ truncateString('Sheng He', 18)}}的其他基金

MEETING: 15th International Congress on Molecular Plant-Microbe Interactions. Kyoto, Japan, - Summer, 2012
会议:第十五届国际分子植物-微生物相互作用大会。
  • 批准号:
    1124968
  • 财政年份:
    2011
  • 资助金额:
    $ 56万
  • 项目类别:
    Standard Grant
Processing of Visual Words in the Human Fusiform Cortex: Selectivity and Plasticity
人类梭状皮层视觉单词的处理:选择性和可塑性
  • 批准号:
    0818588
  • 财政年份:
    2008
  • 资助金额:
    $ 56万
  • 项目类别:
    Continuing Grant
Molecular Basis of Arabidopsis Susceptibility to Pseudomonas Syringae Infection
拟南芥对丁香假单胞菌感染易感性的分子基础
  • 批准号:
    0135286
  • 财政年份:
    2002
  • 资助金额:
    $ 56万
  • 项目类别:
    Continuing Grant
The Rpml Hypersensitive Cell Death Pathway in Arabidopsis Initiated by Pseudomonas syringae AvrB
拟南芥中由丁香假单胞菌 AvrB 启动的 Rpml 超敏细胞死亡途径
  • 批准号:
    9603814
  • 财政年份:
    1998
  • 资助金额:
    $ 56万
  • 项目类别:
    Continuing Grant

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Mapping the molecular basis of growth form divergence in tropical alpine Arabidopsis thaliana
绘制热带高山拟南芥生长形态分化的分子基础
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    418081867
  • 财政年份:
    2019
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研究拟南芥和农作物免疫诱导生长抑制的分子和细胞基础
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    1643005
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The Molecular Basis of Local Adaptation in Arabidopsis thaliana
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