Design of attractors for enhanced sensitivity biosensing

用于增强生物传感灵敏度的吸引子设计

• 批准号: 0507256
• 负责人: Igor Mezic
• 金额: $ 31万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0507256&HistoricalAwards=false
• 关键词: Design; attractors; enhanced; sensitivity; biosensing

We will study nonlinear dynamics of bioparticles at nanoscale for the purpose of design of enhanced sensitivity biosensors. The physical processes that we can improve by pursuing such a study include 1) molecular focusing for purposes of detection and reaction and 2) separation methods for molecular sorting. The essential idea of the proposal is that a combination of hydrodynamic and electromagnetic forces, designed using dynamical systems theory, can enable trapping and separation methods for submicron scale bioparticles. We will focus our study on applications in dielectrophoretic biomolecule manipulation where forces on particles are generated by spatially nonhomogeneous alternating current electric fields and induced fluid flows. However, the dynamical systems aspects of the theory are projected to have broader impact to enabling technologies for manipulation of particles with electromagnetic fields and fluid flows.In particular, major medical technology breakthroughs and advances in security against bioterror will be enabled by the use of electromagnetic fields and fluid flows for manipulation and detection of materials and processes at the nanometer scale. However, the accuracy, speed and sensitivity of the currently available devices is not at the level that allows for such breakthroughs. The lack of quality is in part due to the lack of understanding of nonlinear dynamics of processes and motions at micro- and nanoscale. In our work we will provide such an understanding that will ultimately enable development of fast, miniaturized devices for detection of biotoxins. Our results will also impact developments in the area of point-of-care diagnostics, where medical doctors will be able to perform a simple check based on the "cheek swab" that can tell them whether to administer an antibiotic (and which one, at that) or not in the matter of minutes. The broader impact of the proposed project includes interdisciplinary education of the members of the group. We are planning several educational activities where some of the biotechnology issues that we study will be popularized for K-8 and high-school level students in the region.

我们将研究纳米级生物颗粒的非线性动力学，以设计增强灵敏度的生物传感器。 我们可以通过进行此类研究来改进的物理过程包括 1) 用于检测和反应的分子聚焦以及 2) 用于分子分选的分离方法。 该提案的基本思想是，利用动力系统理论设计的水动力和电磁力的结合，可以实现亚微米级生物颗粒的捕获和分离方法。 我们将重点研究介电泳生物分子操纵中的应用，其中粒子上的力是由空间非均匀交流电场和感应流体流动产生的。 然而，该理论的动力系统方面预计将对利用电磁场和流体流操纵粒子的技术产生更广泛的影响。特别是，通过使用电磁场和流体流在纳米尺度上操纵和检测材料和过程，将实现重大医疗技术突破和生物恐怖安全方面的进步。 然而，当前可用设备的准确性、速度和灵敏度尚未达到允许此类突破的水平。 质量不足的部分原因是缺乏对微米和纳米尺度过程和运动的非线性动力学的理解。 在我们的工作中，我们将提供这样的理解，最终将能够开发出用于检测生物毒素的快速、小型化设备。 我们的结果还将影响现场诊断领域的发展，医生将能够根据“脸颊拭子”进行简单的检查，可以在几分钟内告诉他们是否使用抗生素（以及哪种抗生素）。 拟议项目的更广泛影响包括对小组成员的跨学科教育。 我们正在计划开展多项教育活动，向该地区的 K-8 和高中学生普及我们研究的一些生物技术问题。