RUI: Microfluidic/Capillary Electrophoresis Devices for Chemical Analysis

加州州立大学洛杉矶分校的Frank Gomez教授在分析和表面化学项目的支持下，利用毛细管电泳（CE）和阀门和泵方法开发微流控和非微流控设备，研究受体-配体相互作用。具体来说，工作集中在两个基本项目：微流控装置耦合亲和毛细管电泳（ACE）和微反应器技术在糖肽类抗生素和酶葡萄糖-6-磷酸脱氢酶和脱氢酶的分析。需要解决的问题包括：这组抗生素如何与各种肽相互作用？该项目的目标是自动化研究，用芯片形式的泵和阀门机械取代移液。CE被认为是一种强大的分析分离技术，为复杂混合物的分析提供了快速、定量、重现性和自动化。自动化和减少样品大小的努力将使生物流体和环境样品等复杂混合物的有效分离成为可能。PI在他的研究小组中领导着一群来自不同人群的学生，为他们提供了生物分析、生物化学和仪器开发方面的经验。

Professor Frank Gomez of California State University Los Angeles is supported by the Analytical and Surface Chemistry Program to develop both microfluidic and non-microfluidic devices using capillary electrophoresis (CE) and valve and pump methodologies to study receptor-ligand interactions. Specifically, the work focuses on two basic projects: microfluidic devices coupled to affinity capillary electrophoresis (ACE) and microreactor techniques in the analysis of glycopeptide antibiotics and the enzymes glucose-6-phosphate dehydrogenase and diaphorase. Questions to be addressed include: how does this group of antibiotics interact with various peptides? The project goal is automation of studies, with pump and valve machinery in a chip format replacing pipetting.CE is recognized as a powerful analytical separation technique that offers speed, quantitation, reproducibility, and automation for analysis of complex mixtures. Efforts at automation and reducing sample sizes will allow for efficient separations of complex mixtures such as biological fluids and environmental samples. The PI leads a group of students from diverse populations in his research group, giving them experiences in bioanalysis, biochemistry and instrument development.