RUI: Microfluidic/Capillary Electrophoresis Devices for Chemical Analysis

RUI：用于化学分析的微流体/毛细管电泳装置

• 批准号: 0515363
• 负责人: Frank Gomez
• 金额: $ 45万
• 依托单位: California State L A University Auxiliary Services Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0515363&HistoricalAwards=false
• 关键词: RUI; Microfluidic; Capillary; Electrophoresis; Devices

Professor Frank Gomez of California State University Los Angeles is supported by the Analytical and Surface Chemistry Program to develop both microfluidic and non-microfluidic devices using capillary electrophoresis (CE) and valve and pump methodologies to study receptor-ligand interactions. Specifically, the work focuses on two basic projects: microfluidic devices coupled to affinity capillary electrophoresis (ACE) and microreactor techniques in the analysis of glycopeptide antibiotics and the enzymes glucose-6-phosphate dehydrogenase and diaphorase. Questions to be addressed include: how does this group of antibiotics interact with various peptides? The project goal is automation of studies, with pump and valve machinery in a chip format replacing pipetting.CE is recognized as a powerful analytical separation technique that offers speed, quantitation, reproducibility, and automation for analysis of complex mixtures. Efforts at automation and reducing sample sizes will allow for efficient separations of complex mixtures such as biological fluids and environmental samples. The PI leads a group of students from diverse populations in his research group, giving them experiences in bioanalysis, biochemistry and instrument development.

加州州立大学洛杉矶的Frank Gomez教授得到了分析和表面化学项目的支持，利用毛细管电泳（CE）和阀门和泵方法来研究受体-配体相互作用，以开发微流体和非微流体装置。具体而言，工作重点是两个基本项目：微流体设备耦合到亲和毛细管电泳（ACE）和微反应器技术在糖肽抗生素和葡萄糖-6-磷酸脱氢酶和心肌黄酶的分析。 需要解决的问题包括：这组抗生素如何与各种肽相互作用？ 该项目的目标是实现研究的自动化，用芯片形式的泵和阀门机械取代移液器。CE被公认为一种强大的分析分离技术，可为复杂混合物的分析提供速度，定量，重现性和自动化。 在自动化和减少样品量方面的努力将允许有效地分离复杂的混合物，如生物流体和环境样品。 PI带领一群来自不同人群的学生参加他的研究小组，为他们提供生物分析，生物化学和仪器开发方面的经验。