MRI: Instrument Development of Microfluidic-Based Flow-Injection Capillary Electrophoresis with Fiber-Optics Detection

本仪器建议的广泛目标是开发使用光纤检测的微芯片流动注射毛细管电泳（MFI-CE）。其研究重点是开发和部署用于金属和小分子分析的新分析方法。该仪器开发是化学，生物化学和土木工程专业人员之间的合作。FI-CE在耦合到光纤检测的微流体格式上的集成是独特的。PI之前在微流体格式上设计CE、FI-CE和使用光纤的FI的工作将集成到一个多功能仪器中，该仪器具有小样品体积要求和光纤检测系统的鲁棒性。该仪器最终将用于：1）金属离子分析和形态分析，2）含磷物质分析，3）连续流酶微反应器。这项研究工作将为本科生和硕士生提供一个坚实的背景，在化学和工程的接口检查问题。拟议的仪器项目将使学生参与仪器的设计和开发，并将成为学生广泛教育经验的一部分，包括科学研讨会，指导和课程进修。为参与者计划的研究项目旨在展示技术的跨学科性质及其作为解决广泛科学问题的新的令人兴奋的工具的重要性。总的来说，这个项目将为本科生和硕士生提供坚实的分析化学和工程设计背景，具有广泛的应用。

The broad goal of this instrumentation proposal is to develop microchip flow injection capillary electrophoresis (MFI-CE) using fiber optic detection. Its research focus is on the development and deployment of new analytical methods for the analysis of metals and small molecules. This instrumentation development is a collaboration between PIs in Chemistry, Biochemistry, and Civil Engineering. The integration of FI-CE on a microfluidic format coupled to fiber optic detection is unique. The PIs' previous work designing CE on a microfluidic format, FI-CE, and FI using fiber optics will be integrated into one versatile instrument with small sample volume requirements and the robustness of a fiber optics detection system. The instrument will ultimately be used for: 1) Metal ion analysis and speciation, 2) Phosphorus-containing species analysis, and 3) Continuous flow enzyme microreactors. This research effort will provide undergraduate and masters students a solid background examining problems at the interface of chemistry and engineering. The proposed instrumentation project will involve students in the design and development of the instrument and will be part of a broad educational experience for students that will include scientific seminars, mentoring, and course advisement. The research projects planned for the participants are aimed at demonstrating the interdisciplinary nature of the techniques and its importance as a new and exciting instrument in solving broad scientific problems. Overall, this project will provide undergraduate and masters students a solid analytical chemistry and engineering design background with broad applications.