Microbial Genome Sequencing: Whole Genome Sequencing of Bacillus Megaterium QM B1551

微生物基因组测序：巨大芽孢杆菌 QM B1551 全基因组测序

• 批准号: 0523472
• 负责人: Jacques Ravel
• 金额: $ 63.1万
• 依托单位: J. Craig Venter Institute, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-10-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0523472&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Whole; Bacillus

This goal of this project is to sequence the complete genome of Bacillus megaterium, a bacterium that has a number of unique properties that are of both economic and scientific interest. Its cells are approximately 100 times larger in cell volume than most other bacteria. This increase allows for the examination of cellular localization of certain proteins and protein complexes. A genetic system has been developed and many genetic tools are now available. B. megaterium's ability to synthesize and secrete products without degradation also allows for the large-scale production of beneficial compounds including vitamin B12, antiviral compounds such as oxetanocin, which is active against HIV, and penicillin amidase, used to construct synthetic penicillins. In addition to producing these beneficial compounds, B. megaterium also contains a number of plasmids. These small extrachromosomal pieces of DNA are thought to contain genes responsible for the organism's ability to grow in environmentally contaminated sites as well as aiding in the remediation of these sites. The genetic factors responsible for increased cell size, the process of cell division in this larger bacterium, and the identification of gene products potentially responsible for the remediation of environmentally contaminated sites will be studied through examination of the genome sequence data. B. megaterium is an ancestral relative to other Bacillus species, including the bioweapon pathogen B. anthracis as well as the model bacterial species B. subtilis,. The genome sequencing and comparative genomics of this bacterium may provide additional insights into the development of treatments and therapies for pathogens as well as elucidate basic processes that have been studied in Gram positive bacilli such as sporulation and germination. The project will also include an undergraduate summer workshop, a conference for high school and college general science educators and development of a website to inform the public about the interesting biology of B. megaterium, a charismatic microorganism.

该项目的目标是对巨大芽孢杆菌的完整基因组进行测序，巨大芽孢杆菌是一种具有许多独特性质的细菌，具有经济和科学意义。它的细胞体积大约是大多数其他细菌的100倍。 这种增加允许检查某些蛋白质和蛋白质复合物的细胞定位。 已经开发了一个遗传系统，现在有许多遗传工具。B。巨大芽孢杆菌合成和分泌产物而不降解的能力也允许大规模生产有益的化合物，包括维生素B12、抗病毒化合物如对HIV有活性的氧杂环丁烷和用于构建合成青霉素的青霉素酰胺酶。除了产生这些有益化合物外，B.巨大菌还含有许多质粒。 这些小的染色体外DNA片段被认为含有负责生物体在环境污染场所生长的能力以及帮助修复这些场所的基因。 将通过检查基因组序列数据来研究导致细胞大小增加的遗传因素、这种较大细菌的细胞分裂过程以及可能负责环境污染场地补救的基因产物的鉴定。B。巨大芽孢杆菌是相对于其他芽孢杆菌属物种（包括生物武器病原体B）的祖先。炭疽菌以及模式菌种B。subtilis，这种细菌的基因组测序和比较基因组学可以为病原体的治疗和疗法的发展提供额外的见解，并阐明在革兰氏阳性杆菌中研究的基本过程，如孢子形成和萌发。 该项目还将包括一个本科生暑期讲习班，一个高中和大学普通科学教育工作者会议，以及一个网站的开发，以告知公众关于B有趣的生物学。巨大菌是一种有魅力的微生物