Microbial Genome Sequencing: Insight into the Evolutionary History of Bacilus Subtilis Through Whole Genome Sequencing

这是一个枯草芽孢杆菌群细菌的比较基因组测序项目。 B。枯草芽孢杆菌可分为两个主要亚群：168和W23。迄今为止，唯一可用的基因组序列是168组的代表，已广泛适应实验室条件。这种适应过程，其中包括连续传代和X射线照射，导致了一些生态相关的社会行为的损失，表现出新隔离的环境成员的这个物种。 为了充分了解这一重要模式生物的多样性，将使用全基因组鸟枪测序方法对另外四个B的基因组进行测序。枯草芽孢杆菌分离株：B.枯草芽孢杆菌TU-B-10（W23组的模式菌株）、莫哈韦芽孢杆菌RO-H-1（与小麦枯萎病、烃回收和新的抗真菌特性相关的近亲）、B. subtilis NCIB 3610（B. B. subtilis 168）和B. B. subtilis RO-NN-1（168群中最近的环境分离物，其具有比其它B更大的基因组。枯草杆菌菌株）。枯草芽孢杆菌是继大肠杆菌之后研究最多的微生物。它是超过20，000种科学出版物的主题，也是许多生物过程的模式生物。目前，已有20多个基因组序列可用或正在进行E。大肠杆菌中，只有一个B的基因组序列。枯草杆菌。对这些基因组序列的比较分析将更好地了解这种重要模式生物的进化。更重要的是，大型国际科学界（学术界、工业界和生物防御界）将通过获得这些序列得到更好的服务。该项目还将涉及博士后和本科生一级的重要培训，以及B网站的开发。枯草杆菌比较基因组学

This is a comparative genome sequencing project of bacteria in the Bacillus subtilis group. B. subtilis can be divided into two major subgroups:168 and W23. The only genome sequence available to date is a representative of the 168 group that has been extensively adapted to laboratory conditions. This adaptive process, which includes serial passaging and X-ray irradiation, has resulted in the loss of a number of ecologically-relevant social behaviors exhibited by newly isolated environmental members of this species. To fully appreciate the diversity within this important model organism a whole genome shotgun sequencing approach will be used to sequence the genome of an additional four B. subtilis isolates: B. subtilis TU-B-10 (type strain of the W23 group), Bacillus mojavensis RO-H-1 (close relative associated with wheat blight, hydrocarbon recovery and novel antifungal properties), B. subtilis NCIB3610 (the ancestor to B. subtilis 168) and B. subtilis RO-NN-1 (a recent environmental isolate within the 168 group that has a larger genome than other B. subtilis strains). Bacillus subtilis is the second most studied microbe after Escherichia coli. It is the subject of more that 20,000 scientific publications and a model organism for many biological processes. Currently there are more than 20 genome sequences available or in progress for E. coli and only one genome sequence for B. subtilis. The comparative analysis of these genome sequences will generate a better insight into the evolution of this important model organism. More significantly, the large and international scientific community (academic,industrial and biodefense) will be better served by having access to these sequences. The project will also involve significant training at the postdoctoral and undergraduate levels, and the development of a website of B. subtilis comparative genomics.